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Chinese Journal of Virology ; (6): 97-102, 2011.
Artículo en Chino | WPRIM | ID: wpr-286070

RESUMEN

In 2006, the first Chinese Tahyna virus isolate (XJ0625) was obtained in Xinjiang province and human infection were found in the same region. In this study, cell culture, animal experiments, electron microscopy, immunofluorescence assay and cross neutralization tests were performed to see the cell susceptibility, animal pathogenicity, morphology and antigenic and other biological characteristics of XJ0625. In addition, molecular biology software was used to analyze the characteristics of molecular evolution. The results showed that BHK-21 cell line was susceptible to XJ0625 and the virus was lethal to suckling mice when injected by intracranial ways. Similar to the other Bunyavirus, Tahyna virus is spherical enveloped virus under electron microscopy. XJ0625 infected cells showed strong fluorescent signal and could be neutralized by immune asities fluid with immnity to protype Tahyna virus Bardos 92. The sequence of the S and M segments showed 91.8% and 81.9% homology with Bardos 92.


Asunto(s)
Animales , Femenino , Ratones , Línea Celular , China , Virus de la Encefalitis de California , Genética , Fisiología , Evolución Molecular , Técnica del Anticuerpo Fluorescente , Sistema Nervioso , Virología , Filogenia , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN
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