RESUMEN
Objective To evaluate the influence of sample collection and storage on the detection results of intact parathyroid hormone(iPTH).Methods 10 blood samples from the hemodialysis patients were simultaneously injected into four types of blood collection tube:pro-coagulation tube,blank tube,EDTA tube and sodium citrate tube.The iPTH levels in the fresh samples within 4 h after blood collection and serum/plasma preserving for 12,24,48 h under 4OC were detected by the Roche cobase411 immunoas-say instrument.Results Serum iPTH in the pro-coagulation tube within 4 h after blood collection was rapidly degraded,while the iPTH level in the blank tube was highest,which was significantly higher than that the serum/plasma preserved for 12,24,48 h.The iPTH level in the EDTA tube and the sodium citrate tube began to degrade only at 48 h.Conclusion It is the first time to reveal that the coagulant has the degrading or inhibiting effect on serum iPTH,which verifies that the EDTA tube is the optimal blood col-lection tube.
RESUMEN
Objective:hepaCAM (hepatocyte cell adhesion molecule)is a kind of immunoglobulin superfamily member which was discovered as a tumor suppressor. This study was to investigate the effects of hepaCAM transfection on proliferation and invasion of human renal cancer cell line 786-0. Methods:An eukaryotic expression plasmid containing hepaCAM,hepaCAM-pEGFPN2 was transfected into 786-0 cells,then positive cell clones were selected with G418 and amplified. pEGFPN2 transfected cells and untreated cells were set as controls.The expression of hepaCAM was detected by reverse transcription-polymerase chain reaction (RT-PCR). Proliferative ability of 786-0 cells was measured by MTT assay and the invasive ability in vitro was determined by transwell chambers method. Results: Plasmid pEGFPN2,hepaCAM-pEGFPN2 were successfully constructed and stably transfected into 786-0 cells.After transfection,the inhibitory rates of cell growth at the fourth、 fifth and sixth day were 26.5%,38.1% and 35.7%,respectively. The penetrating cells were significantly less in 786-0/ hepaCAM-pEGFPN2 group than in 786-0/pEGFPN2 and 786-0 groups (24.6?5.3vs35.5?6.2 and 37.1?7.0,P