Rehabilitation effect of Baduanjin exercises combined with stretch training with band for elderly patients with sarcopen / 国际中医中药杂志

Objective:To observe the rehabilitation effect of Baduanjin combined with elastic band training on elderly patients with sarcopenia. Methods:From January 2019 to January 2021, 120 elderly sarcopenia patients in our hospital who met the inclusion criteria were divided into two groups according to the random number table method, with 60 in each group. The control group was given conventional treatment intervention for sarcopenia, and the observation group was Baduanjin exercises combined with elastic band training on the basis of the control group. Both groups were intervened for 12 weeks. The skeletal muscle index (SMI), muscle grip strength, short physical performance battery (SPPB) and Modified Barthel Index (MBI) were used to compare between two groups before and after the intervention. Results:After treatment, the SMI index [(6.77 ± 1.03) kg/m 2vs. (6.35 ± 1.12) kg/m 2, t=2.14], muscle grip strength [(23.06 ± 3.48) kg vs. (19.41 ± 3.79) kg, t=5.50], SPPB score [(9.12 ± 2.24) vs. (7.85 ± 2.13), t=3.18] and MBI score [(82.43 ± 20.75) vs. (64.36 ± 19.42), t=4.93] of the observation group were significantly higher than those in the control group ( P<0.05). After treatment, the distribution of muscle activity function in the observation group was significantly better than that of the control group ( Z=-2.28, P=0.023). Conclusion:Baduanjin combined with elastic band training can improve the muscle function and quality of daily activities in elderly patients with sarcopenia.

miR-575 Promotes the Proliferation and Invasion of Non-small Cell Lung Cancer Cell by Negatively Regulating BLID Expression / 现代生物医学进展

Objective:To explore the mechanisms of regulation of miR-575 on the proliferation and invasion properties of non-small cell lung cancer cell (NSCLC).Methods:Real-time PCR was selected to detect the expression of miR-575 and BLID in differ ent NSCLC cell lines.CCK-8 assay was processed to measure the alternations of A549 cell proliferation at different time points after transfection of miR-575 mimic and miR-575 inhibitor.The invasion ability of A549 cells was evaluated by transwell.The targeting of BLID by miR-575 was predicted by Targetcan software and verified by dual-Luciferase assay.BLID protein expression level was detected by western blot.Results:miR-575 highly expressed in NSCLC cell lines,including A549,SPC-A1,H1299,H1650 (P＜0.001),miR-575 mimic could efficiently elevated the expression ofmiR-575 in A549 cells (P＜0.001),and strengthened the proliferation and invasion ability of NSCLC cells (P＜0.05),while,transfection of miR-575 inhibitor could down-regulate the expression ofmiR-575,and also inhibit the proliferation and invasion ability of NSCLC cells (P＜0.01).Targetscan software predicted that BLID might be the target gene of miR-575,and dual-luciferase assay revealed that miR-575 could obviously decrease the luciferase reaction of wild type BLID 3'UTR (P＜0.01),besides,miR-575 could down-regulate the protein expression ofBLID (P＜0.01).Real-time PCR results showed that NSCLC cell lines had lower level of BLID mRNA expression compared with 16HBE control cells (P＜0.001),and restore of BLID could markedly inhibited cell proliferation and invasion ability (P＜0.05),which could be reversed by miR-575 co-tranfection (P＜0.01).Conclusion:In NSCLC cells,the expression ofmiR-575 could promote cell proliferation and invasion ability by directly regulating downstream target tumor-suppressor gene BLID expression.

SU11274 reverse geiftinib resistance induced by hepatocyte growth factor in differentEGFR gene type of non-small cell lung cancer cells / 中国癌症杂志

Background and purpose:Hepatocyte growth factor (HGF) induce epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) resistance in non-small cell lung cancer (NSCLC) cells, the mechanism might be related with activation of c-Met. The present study aimed to explore whether c-Met inhibitor SU11274 reverse gefitinib resistance induced by HGF in differentEGFR gene types of NSCLC.Methods:PC9 (EGFR-activating mutant), H292 (EGFR-wild type) and A549 (EGFR-wiled) were chosen. The experiments were divided into 6 groups:C group (control), H group (HGF), G group (geiftinib), S group (SU11274), GH group (geiftinib+HGF), GSH group (geiftinib+SU11274+HGF). The cell survival was measured by MTT assay; the cell apoptosis was measured by lfow cytometry (FCM); the expressions of c-Met, Stat3, Akt and Erk1/2 protein were examined by Western blot.Results:Gefitinib inhibited cell growth of 3 cells lines in a dose-dependent manner, and treating with HGF could relieve inhibition of cell growth caused by geiftinib. The cell survival when treating the HGF-induced cell lines with defferent concentration of geiftinib combined with SU11274 was signiifcantly decreased than that when treating HGF-induced cell lines with geiftinib alone. In 3 cell lines, the apoptosis rate in HGS group was higher than that in HG group (P<0.05). In three cells lines, the p-Met, p-Stat3, p-Akt and p-Erk1/2 expressions in HGS group were lower than that in HG group (P<0.05).Conclusion:SU11274 reversed geiftinib resistance induced by HGF in different EGFR gene types of NSCLC cells, the mechanism might be related with inhibiting the HGF-induced activation of c-Met and its downstream signaling pathway.

Early intensive blood pressure reduction in acute cerebral hemorrhage / 国际脑血管病杂志

There are more prognostic factors affecting the patients with cerebral hemorrhage, including bleeding volume, bleeding site, cause of bleeding, and blood pressure regulation during acute phase. Among them, the regulation of blood pressure during acute phase is a man-controlled important factor. However, the ideal blood pressure level has not yet to he determined at present. Therefore, more clinical trials are needed to determine the exact time window of blood pressure management in patients with intracerebral hemorrhage during acute phase and the range of blood pressure control.