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Chinese Journal of Biotechnology ; (12): 2008-2013, 2009.
Artículo en Chino | WPRIM | ID: wpr-336274

RESUMEN

In order to enhance the utilization efficiency, reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration; we studied the ligninase production by Coprinus comatus, Aspergillus niger and Trichoderma reesei through the plate screening. The results showed that C. comatus mixed culture with T. reesei have a good compatibility and higher yields of Laccase. On the basis of this pre-experiment, we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions: the inoculation proportion of C. comatus and T. reesei (5:2), the interval of time (12 h), the temperature 260C, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.


Asunto(s)
Coprinus , Metabolismo , Técnicas de Cultivo , Métodos , Fermentación , Oxigenasas , Tallos de la Planta , Metabolismo , Trichoderma , Metabolismo , Zea mays , Metabolismo
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