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1.
Journal of Central South University(Medical Sciences) ; (12): 170-173, 2011.
Artículo en Chino | WPRIM | ID: wpr-814587

RESUMEN

OBJECTIVE@#To evaluate the clinical therapeutic effect of Rabdosia rubescens aqueous extract on chronic pharyngitis and its safety.@*METHODS@#One hundred and two patients suffered from chronic pharyngtis were randomly divided into an interventional group (n=51) and a placebo group (n=51). These volunteers were given 220 mL test materials once a day double-blindly for 30 days. All patients took routine physical examinations, and their throat symptom and pharynx signs were observed before and after the experiment.@*RESULTS@#After the patients had consumed Rabdosia rubescens aqueous extract and the placebo for 30 days, the improvement rates of main clinical symptom and signs in the interventional group were 67.31% and 61.54%, while those in placebo group were 19.23% and 11.54%, with significant difference (P0.05). But after 30 days, the integral of main clinical symptom and signs in the interventional group significantly decreased (P<0.05), compared with itself before the experiment and the placebo group.@*CONCLUSION@#Rabdosia rubescens aqueous extract is safe and effective for patients with chronic pharyngitis.


Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedad Crónica , Método Doble Ciego , Medicamentos Herbarios Chinos , Usos Terapéuticos , Isodon , Química , Faringitis , Quimioterapia , Fitoterapia
2.
Chinese Journal of Biotechnology ; (12): 247-252, 2011.
Artículo en Chino | WPRIM | ID: wpr-324556

RESUMEN

pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.


Asunto(s)
Humanos , ADN Recombinante , ADN Superhelicoidal , Escherichia coli , Genética , Metabolismo , Fermentación , Vectores Genéticos , Genética , Factor de Crecimiento de Hepatocito , Genética , Proyectos Piloto , Plásmidos
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