Association between HIV and proven viral lower respiratory tract infection in paediatric intensive care unit (PICU) patients at Inkosi Albert Luthuli Central Hospital (IALCH), Durban, South Africa

Acute viral respiratory infections are common within the paediatric population.Nucleic Acid amplification tests can identify a wide range of respiratory viruses. Virally infected patients can now be diagnosed early and more accurately in the acute phase of illness.Objectives:The objective was to examine the association between HIV status and mortality in children with viral lower respiratory tract infection (LRTI) and to delineate the profile of viruses identified.Methods:A retrospective review of charts of children aged from birth to 10yrs of age admitted to PICU at IALCH with a viral lower respiratory tract infection from December 2010 to May 2015 was conducted. Only patients who had a positive respiratory viral multiplex test were eligible for entry into the study. Patients were divided into HIV positive; and negative; patients and mortality in the two groups was assessed.Results:Three hundred and thirty eight records were entered into the study. Sixty five patients tested HIV positive (19.23%) and 273 tested HIV negative (80.77%).There was a total of 55 deaths among the charts that were reviewed. Of these 55 deaths, 12 were amongst the 65 HIV positive patients (18.46%), and 43 amongst the 273 HIV negative patients (15.75%). The difference in mortality according to HIV status was not statistically different (p = 0.595).RSV was the most prevalent virus identified overall, with adenovirus being most prevalent in the HIV positive group.Conclusion:The results showed that patients with viral LRTI requiring respirato

PIMAPotassium Iodide Point-of-Care Testing for CD4 Counts in Predicting Antiretroviral Initiation in HIV-Infected Individuals in KwaZulu-Natal; Durban; South Africa

Introduction: Limited information is available on the usefulness of the PIMATM analyser in predicting antiretroviral treatment eligibility and outcome in a primary healthcare clinic setting in disadvantaged communities in KwaZulu-Natal; South Africa.Materials and methods: The study was conducted under the eThekwini Health Unit; Durban; KwaZulu-Natal. Comparison of the enumeration of CD4+ T-cells in 268 patients using the PIMATM analyser and the predicate National Health Laboratory Services (NHLS) was undertaken during January to July 2013. Bland-Altman analysis to calculate bias and limits of agreement; precision and levels of clinical misclassification at various CD4+ T-cell count thresholds was performed.Results: There was high precision of the PIMATM control bead cartridges with low and normal CD4+ T-cell counts using three different PIMATM analysers (%CV 5). Under World Health Organization (WHO) guidelines (