Native and oxidized low density and very low density lipoprotein enhance the expression of MIP-1? mRNA in aortic smooth muscle cells / 中国病理生理杂志

AIM: To understand whether native and oxidized low density and very low density lipoprotein (n-LDL, n-VLDL, ox-LDL, ox-VLDL) enhance the expression of macrophage inflammatory protein (MIP)1? mRNA in cultured aortic smooth muscle cells (SMCs). METHODS: Native low density and very low density lipoprotein were isolated from normal blood donors by density gradient ultracentrifugation, and were oxidatively modified by adding CuCl 2. After a 24 h-exposure of the cultured SMCs to n-LDL, n-VLDL, ox-LDL and ox-VLDL, respectively, the expression of MIP-1? mRNA was determined by in situ hybridization and RT-PCR. RESULTS: Cultured aortic SMCs expressed MIP-1? mRNA at low level. N-LDL, n-VLDL, ox-LDL and ox-VLDL enhanced the expression of MIP-1? mRNA in SMCs, ox-LDL and ox-VLDL showed stronger effect than n-LDL and n-VLDL, respectively. The effect of ox-VLDL was most striking. There was a significant difference between groups ( P

Ultrastructural changes of gastroduodenal mucosa after severe burns in rats / 第三军医大学学报

usually in the 24th to 72nd h postburn.There was edema associated with mild necrosis of the duodenal mucosa immediately after burns.Severe necrosis occurred in the 2nd h postburn.The manifestations reached the peak in the 24th h and were alleviated in the 72nd h postburn.As a rule,the damages on the duodenal mucosa occurred early and persisted for a long period with high incidence and severe wounding conditions.The damages of gastroduodenal mucosa were more severe in the phosphorus burn group than in the napalm burn proup mainly because of the more intense heat stress after phosphorus burns and the tosicity of the element phosphorus and phosphorus free redicals.