Determination of anthraquinone in Semen Cassiae from different regions by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the content of 7 anthraquinones in Semen Cassiae.</p><p><b>METHOD</b>A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).</p><p><b>RESULT</b>Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.</p><p><b>CONCLUSION</b>The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.</p>