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Objective To investigate the relationships between the expression levels of tumor necrosis factor receptor associated factor 4 (TRAF4) and ribosomal S6 protein kinase 4 (RSK4) protein in gastric cancer tissues and the recurrence after laparoscopic radical gastrectomy. Methods In total, 176 patients were divided into the recurrence and non-recurrence group, and the expression levels of TRAF4 and RSK4 protein in cancer and adjacent tissues and in gastric cancer tissues in the recurrence and non-recurrence group were compared. The influencing factor of recurrence and the efficacy of TRAF4 and RSK4 protein expression in predicting recurrence were analyzed. Results The positive expression rate of TRAF4 protein in gastric cancer tissues was higher than that in adjacent tissues (P < 0.05) and that in the recurrence group was higher than that in the non-recurrence group (P < 0.05). The positive expression rate of RSK4 protein in gastric cancer tissues was lower than that in adjacent tissues (P < 0.05) and that in the recurrence group was lower than that in non-recurrence group (P < 0.05). The largest tumor diameter 5 cm, poor differentiation, TNM Ⅲ stage, depth of invasion T3-T4, lymph node metastasis, absence of adjuvant chemotherapy after operation, positive expression of TRAF4 and RSK4 protein, and regular diet w influenced the post-operative recurrence (all P < 0.05). The accuracy of TRAF4 and RSK4 protein in gastric cancer tissues in combined predicting the recurrence was 83.52%. Conclusion The expression of TRAF4 protein is high, and the RSK4 protein is low in gastric cancer tissue, which are related to recurrence.
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In recent years, new targets research of Anti-HIV drugs brings in new hope for the development of Anti-HIV drags. Based on Science Citation Index Expanded, the paper analyzes the research focus, country distribution and research institution distribution of new targets of anti-HIV drugs by Bibliometrics.
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Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
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Citrullus , Genética , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Genes Reporteros , Vectores Genéticos , Genética , Proteínas Fluorescentes Verdes , Genética , Metabolismo , Sustancias Luminiscentes , Metabolismo , Proteínas Luminiscentes , Genética , Metabolismo , Solanum lycopersicum , Genética , Regiones Promotoras GenéticasRESUMEN
More than 100 countries have added hepatitis B vaccination to their routine immunization programmes.Hepatitis B vaccines progressed from blood vaccines to recombinant vaccines,then to peptide vaccines and DNA vaccines,to their functions extended from prophylactic to therapeutic.This article analyzes the relationship between hepatitis B vaccine R&D and the immunization programmes in different countries,to summarizes the R&D of both prophylactic and therapeutic vaccines,and points out the direction for futare vaccine development.