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Objective To study the effect of Chinese traditional medicines on human hypertrophic scar fibroblasts in vitro,and determine their effective dosage.Methods Traditional Chinese medicines were cultured with human hypertrophic scar fibrob lasts(Fb)and normal dermal Fb,MTT colorimetr ic assay and cell growth curve were used to compare the influence of ethanol extracts fromChinese tradi tional medicines on the Fb growth activity and growth cycle.Results(1)Chinese gall markedly inhibited the proliferation of Fb derived from hyp ertrophic scar and normal skin,and was more effective on the former in a dose-dependent m anner(0～400?g /mL)during 72h of treatment.(2)The half inhibition concentration o f Chinese gall ethanol extract was 100?g /mL.The morphology of Fb did not change when its concentration was below 400?g /mL,as compared to colchicine(
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Objective To investigate the effects of gallotannic acid, the alcohol extract of Galla chinensis, the alcohol extract of Flos carthami and the water extract of Aloe vera on normal human keratinocytes, melanocytes and fibroblasts in vitro, and get a better understanding of the mechanism of action and the possible adverse effects of Galla chinensis in treating keloid. Methods MTT colorimetric assay was used to detect the effects of gallotannin, colchicine and the later two extracts on the growth activity of the different cells. Results ①The inhibitory action of gallotannin to fibroblasts proliferation was similar to that of the alcoholic extract of Galla chinensis, and the inhibitory action of gallotannin at ≥ 20 ?g/mL to the proliferation of melanocytes was obvious (the proliferation rate was 73.6% - 68% of untreated cells), while gallotannin at ≤ 50 ?g/mL slightly enhanced the growth activity of keratinocytes (the proliferation rate was 111% - 112% of untreated cells). ②Both the alcoholic extract of Flos carthami and the water extract of Aloe vera enhanced the proliferation of keratinocytes in a dose-dependent mode. The water extract of Aloe vera also enhanced the proliferation of fibroblasts. Conclusions The gallotannin may be the main pharmacologically active component of Galla Chinensis for treating keloid, and its inhibitory activity to human melanocytes may implicate its potential adverse effects on the skin.
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0.05).③ HDF feeder layer could improve the efficient growth of primary keratinocyte culture plated at very low seeding densities (0.1? 104 cells/cm2). Conclusions Different allogenic keratinocytes can grow in HDF feeder layers from various passages of the same HDF line. HDF feeder layer is able to improve the seeding efficacy of human keratinocytes, which may be the reason te culture plated at very low seeding