RESUMEN
Objective: To evaluate the effect of Haima Bushen Pills (HBP) on nourishing yin and reinforcing kidney, and study the mechanism of the action with aim to provide the experimental evidence for the clinic. Methods: Rats model with kidney-yang deficiency was induced by hydrocortisone through intramuscular injection to observe the anti kidney-yang deficiency effect of HBP. The effect of HBP against kidney-yin deficiency was observed using the kidney-yin deficiency model induced by oral administration of thyroxine and reserpine. The effect of HBP on erectile function was observed by intramuscular injection of hydrocortisone in rats. Castrated male rats and normal young male rats were administered orally to observe the androgenic effect of HBP. Results: After 10 d of administration, HBP at dose of 1.1 g/kg and 2.2 g/kg significantly increased the number of autonomic activities of kidney-yang deficiency mice, extended the duration of low-temperature swimming, and increased the organ coefficients of thymus and spleen. It also significantly reduced the number of platform jumping errors and prolonged the incubation period of errors, significantly increased the pain threshold and increased the organ coefficients of thymus and spleen in mice with kidney-yin deficiency. After 2 weeks of administration, HBP at doses of 0.56 g/kg and 1.12 g/kg significantly increased the bR/rR ratio and intravitreal pressure in rats, significantly improved the erectile function, significantly increased the serum testosterone (T) concentration and the viscera coefficients of prostate, foreskin gland and levator ANI of castrated rats. The serum concentration of testosterone (T), follicle-stimulating hormone (FSH) and viscera index of seminal vesicle were significantly increased in normal young rats after administration of HBP. Conclusion: HBP has obvious effect on nourishing yin and reinforcing kidney.