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1.
Chinese Journal of Plastic Surgery ; (6): 47-48, 2006.
Artículo en Chino | WPRIM | ID: wpr-240387

RESUMEN

<p><b>OBJECTIVE</b>To evaluate the aesthetic outcome after application of rectangle periosteous flap to cover corrugator.</p><p><b>METHODS</b>On the basis of regular rhytidectomy, a inter-eyebrow periosteous flap is applied to cover the fascia flap of corrugator by means of turnover. This manipulation helps to separate the skin from the muscle and prevent the re-adherence between the skin and muscle.</p><p><b>RESULTS</b>The approach was applied on 15 cases. The follow-up ranged from 6 months to 1 year. The results were satisfactory.</p><p><b>CONCLUSIONS</b>The method is effective in elimination of inter-eyebrow crease.</p>


Asunto(s)
Adulto , Femenino , Humanos , Persona de Mediana Edad , Músculo Esquelético , Trasplante , Periostio , Trasplante , Procedimientos de Cirugía Plástica , Métodos , Ritidoplastia , Métodos , Cráneo , Colgajos Quirúrgicos , Resultado del Tratamiento
2.
Chinese Journal of Biotechnology ; (12): 365-368, 2002.
Artículo en Chino | WPRIM | ID: wpr-256201

RESUMEN

Canine Interferon-gamma (CaIFN-gamma) cDNA was cloned from spleen T cells of dog by reverse transcription-polymerase chain reaction (RT-PCR). CaIFN-gamma cDNA were digested with Hind III and Not I, and inserted into pRc/CMV2 expression vector. The pRc/CMV2/CaIFN-gamma vector was sequenced, and predicted to produce a signal peptide of 23 amino acids and a mature protein of 143 amino acids with a molecular weight of 19 kD. Two potential N-glycosylation sites are located at positions 16 and 83 of the mature protein. Comparison of the CaIFN-gamma protein sequence with that of CaIFN-gamma reported from DDBJ/GenBank revealed a homology of 99%. To establish a long time expression system, pRc/CMV2/CaIFN-gamma vector was transfected into mouse SP2/0 cell line. The SP2/0 cells culture supernatants was harvested and the antiviral activity was measured following cytopathic-effect inhibition assay using Madin-Darby Canine Kidney (MDCK)-vesicular stomatitis virus(VSV) system. Initial transformants with G418 phenotype produced recombinant CaIFN-gamma titers ranging from 2,500 to 5,000 u/mL of culture medium.


Asunto(s)
Animales , Bovinos , Perros , Ratones , Secuencia de Aminoácidos , Secuencia de Bases , Neoplasias de la Médula Ósea , Metabolismo , Línea Celular , Clonación Molecular , ADN Complementario , Química , Interferón gamma , Genética , Farmacología , Datos de Secuencia Molecular , Proteínas Recombinantes
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