Yiqixue buganshen recipe regulates the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice / 中国结合医学杂志

<p><b>OBJECTIVE</b>To observe the effect of Yiqixue Buganshen recipe(, YBR) on the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice.</p><p><b>METHODS</b>A total of 180 mice were divided into three groups: model group, treatment group and control group. The treatment and model groups were intraperitoneally injected with gonadotropin-releasing hormone analogue for 7 days; pregnant mare serum gonadotropin was also injected on the 7th day. After 48 h, human chorionic gonadotropin was injected. The control group was injected with an equal volume of saline at the same time. From the start of the experiment, the treatment group was intragastrically administered Jinghouzengzhi Recipe() and Cuhuangti Recipe(). The model group and the control group were intragastrically administered an equal volume of saline. Real-time reverse transcription polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression of integrin ανβ3 in mouse endometrium.</p><p><b>RESULTS</b>Integrin ανβ3 was expressed in mouse endometrium in all groups. Integrin αββ3 expression increased gradually along with pregnancy, progressing from pregnant day (Pd) 1. Integrin ανβ3 expression significantly increased on Pd 4, then began to decrease on Pd 6. Integrin ανβ3 expression in the treatment group was higher than in the model group, and the difference was statistically significant (P <0.05). The difference between the treatment group and the control group was not statistically significant (P >0.05).</p><p><b>CONCLUSION</b>YBR improves endometrial receptivity, and may play an important role in embryonic implantation.</p>

Fertilization rates following 109 half intracytoplasmic sperm injection cycles for in vitro fertilization-embryo transfer / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the clinical outcomes of half intracytoplasmic sperm injection (partial ICSI) treatment in infertile patients with potential fertilization failure.</p><p><b>METHODS</b>A total of 109 partial ICSI cycles of in vitro fertilization-embryo transfer (IVF-ET) were classified into 5 groups, namely group A (infertile patients for unidentified causes, 17 cycles), group B (oligo-asthenozoospermia patients, 28 cycles), group C (teratozoospermia patients, 8 cycles), group D (primary infertile patients without definite causes, 31 cycles), and group E (secondary infertile patients without definite causes, 25 cycles). The fertilization rate and normal fertilization rate after IVF and ICSI were compared between the groups.</p><p><b>RESULTS</b>Significant differences were found in the fertilization rate following conventional IVF and ICSI in group A (53.1-/+38.8% vs 72.2-/+34.1%) and group D (58.8-/+31.6% vs 82.7-/+21.4%) (P<0.05), but not in groups B, C and E (P>0.05). The normal fertilization rates following IVF and ICSI in groups A, B, D, E were statistically different (P<0.05), but similar in group C (P>0.05).</p><p><b>CONCLUSION</b>ICSI treatment may increase the fertilization rate of IVF-ET in patients with unexplained infertility and primary infertility, but not in patients with oligo-asthenozoospermia, teratozoospermia or secondary infertility.</p>