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Objective To investigate the correlation between microvessel density (MVD) and expression of NET-1 protein in bladder carcinoma and its prognostic significance. Methods The expression NET-1 protein and MVD marked with CD_ 105 in 43 cases of bladder carcinoma and 10 normal bladder tissues were measured by means of immunohistochemistry SP.The association between them and the relation ship with WHO pathology classification,TNM stage and prognosis were studied. Results MVD were correlated with WHO pathology classification,TNM stage and prognosis (F_1=17.01,F_2=12.06,P
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OBJECTIVE To investigate the ESBLs produced by clinical strains of Enterobacter cloacae.METHODS Production of ESBLs was identified with modification of the double-disk test(MDDT).Eight kinds of primers(CTXM,CTXM-1,CTX-M-2,CTX-M-9,SHV,TEM,VEB,and PER)were used for the PCR amplification.Gene clone and DNA sequencing were performed then.RESULTS The result of MDDT was positive,amplicons were gained by PCR amplification with CTXM-1.DNA sequencing of amplicons of this strain revealed ESBLs of CTX-M-3.CONCLUSIONS ESBLs are the important reason for E.cloacae resisting to the third-generation cephalosporins.