RESUMEN
Our knowledge of molecular events and interactions in cell biology and molecular biology are mostly based on findings in nonliving body condition while ignoring the whole process of the events,which inevitably involves false assumption that all molecules behave in the same environment and in the same way. With the emergence of new fluorescent molecular probes prepared through linking new fluorescent material such as quantum dots (QDs) to micromolecule antibody such as antibody simulacrum, the development of methods for transducing extrinsic fluorescent probes into live cells,the application of laser technology, the finer fluorescent micro-imaging system, and the fast and sensitive fluorescence collection system in the application of life sciences, it is now possible to image the trajectory, kinetic behavior, and interaction of biomolecules in live cells. Visualizing single molecule in live cells has opened a new chapter for cell biology and molecular biology. Recent research developments and foreground of single molecule visualizing are discussed in this article.
RESUMEN
Objective:To study the biocompatibility of semiconductor quantum dots to the rat dental papillae cells(RDPCs) cultured in vitro. Methods:①RDPCs isolated and cultured with enzyme digestion. Wave form fibroin and cytokeratin were used to identify the cells resource and the growth rules of cells was observed.②To study the effeel of QDs to the RDPCs,The RDPCs were cultured with the 605QDs,525QDs and those mixture in different density.③With the MTT, to studv the cytotoxicity of QDs to the RDPCs,The RDPCs were cultured with the 605QDs,525QDs and those mixture in different density. Results:①RDPCs cultured in vitro were well growing. Wave form fibroin staining was positive and cytokeratin staining was negative in RDPCs.②The 605QDs,525QDs and those mixture in different density show no negative effect and cytotoxicity on the growth of the RDPCs. Conclusion:The 6050Ds、525QDs in a range of density have well biocmpatibility to the live cells,which supplied scientific evidence for the research of multichannel signal in living cells in vivo physiological condition