Comparison of Advanced Platelet Rich Fibrin (A-PRF) and Culture Media Conditioned Warton's Jelly (CMCWJ) on Fibroblast Cells Proliferation

Objective: To compare the potency of fibroblast cells proliferation in 12.5% and 25% Culture Media Conditioned Warton's Jelly (CMCWJ) and Advanced Platelet Rich Fibrin (A-PRF) cultured medium. Material and Methods: Fibroblast cells were divided into five groups: Group I (Control Group): serum-starved fibroblast without any treatment as a negative control; Group II: fibrolast that supplemented in 12.5% CMCWJ medium; Group III: fibrolast that supplemented 12.5% A-PRF medium; Group IV: fibrolast that supplemented 25% CMCWJ medium, and Group V: fibrolast that supplemented 25% A-PRF medium. The fibroblasts proliferation was counted by an automated cell counter. Statistical analysis was performed using One-way ANOVA and Post hoc Tamhane test was conducted to analyze the potential fibroblast proliferation differences in different concentration of CMCWJ and A-PRF group. Results: There were no significant differences in the fibroblast cell proliferation between GI and GIV, GII and GIV, GII and GIII, GII and GV, also GIV and GV. There were significant differences between GI and GII, GI and GIII, GI and GV, also GIII and GIV. Conclusion: The 12.5% CMCWJ group, 12.5% A-PRF group and 25% A-PRF group has excellent potential ability of fibroblast cells proliferation, meanwhile 25% CMCWJ group has the lowest mean potency of fibroblast cells proliferation compared to other groups. The 12.5% A-PRF Group has the highest mean of fibroblast cell proliferation amongst other groups.

Genotyping the G types of rotavirus and its clinical presentation in children under five years old with diarrhea in the government clinics in Pekanbaru, Indonesia

Aims@# Acute diarrhea is still one of the major causes of death in children under five years old worldwide, including Indonesia. Data from the World Health Organization (WHO) Southeast Asia Region shows that acute diarrhea has caused approximately 10-11% of death in children under five years old and rotavirus (RV) is one of the major causes. This study is conducted to identify the profile and P genotypes of RV among children with acute diarrhea in Pekanbaru.@*Methodology and result@#Descriptive cross sectional study was conducted in children within the range of age 0-60 months with acute diarrhea who admitted to the government clinics and General Hospital of Riau Province from January to July 2015. Immunochrommatography and RT-PCR were used to identify the P genotype of RV from the stool specimens. The profile of patients was investigated including age, sex, hydration and nutritional status, also the exclusive breastfeeding. There were 71 children who suffered from acute diarrhea and 62.0% of stools specimens were positive by RT-PCR for RV infection. The highest rate was in the age group of 6-35 month (70.5%). Most of the patients were female (54.5%), the history of exclusive breastfeeding (54.5%), with moderate dehydration (54.5%) and good nutritional status (97.7%). The RV genotyping results showed the highest proportion was found in the P4 genotype (31.8%), followed by the P8 and P6 genotype, respectively 18.2% and 13.6%, P9 (11.4%), P10 (9.1%), P11 (2.3%) and indeterminate genotype (13.6%).@*Conclusion, significance and impact of study@#Rotavirus was found to be a considerable role as the etiology of diarrhea in children under five years in Pekanbaru and rotavirus P4 genotype was predominant. The result of this study is important for designing antigen-antibody diagnostics against RV.

Identification of the P Genotypes of rotavirus in children with acute diarrhea in Pekanbaru, Indonesia

Aims: Acute diarrhea is still one of the major causes of death in children under five years old worldwide, including Indonesia. Data from the World Health Organization (WHO) Southeast Asia Region shows that acute diarrhea has caused approximately 10-11% of death in children under five years old and rotavirus (RV) is one of the major causes. This study is conducted to identify the profile and P genotypes of RV among children with acute diarrhea in Pekanbaru. Methodology and results: Descriptive cross sectional study was conducted in children within the range of age 0-60 months with acute diarrhea who admitted to the government clinics and General Hospital of Riau Province from January to July 2015. Immunochrommatography and RT-PCR were used to identify the P genotype of RV from the stool specimens. The profile of patients was investigated including age, sex, hydration and nutritional status, also the exclusive breastfeeding. There were 71 children who suffered from acute diarrhea and 62.0% of stools specimens were positive by RT-PCR for RV infection. The highest rate was in the age group of 6-35 month (70.5%). Most of the patients were female (54.5%), the history of exclusive breastfeeding (54.5%), with moderate dehydration (54.5%) and good nutritional status (97.7%). The RV genotyping results showed the highest proportion was found in the P4 genotype (31.8%), followed by the P8 and P6 genotype, respectively 18.2% and 13.6%, P9 (11.4%), P10 (9.1%), P11 (2.3%) and indeterminate genotype (13.6%). Conclusion, significance and impact of study: Rotavirus was found to be a considerable role as the etiology of diarrhea in children under five years in Pekanbaru and rotavirus P4 genotype was predominant. The result of this study is important for designing antigen-antibody diagnostics against RV.