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Objective To explore the application of left colon artery and retention of anal canal in rectal cancer anterior resection .Methods 134 patients with rectal resection were divided into two groups by random number table.The observation group (n=67) received anterior resection of the left colonic artery and retained anorectal anterior resection.The control group(n=67) received subconjunctival artery root ligation ,non -retained anorectal anterior resection of rectal cancer .The number of lymph node dissection and the incidence of postoperative anastomotic leakage were compared between the two groups .The rate of reoperation was compared between the two groups .Results The number of lymph node dissection in the observation group and control group was (4.2 ±1.3),(4.4 ±1.2),the difference was not statistically significant (P=0.450).The incidence rate of anastomotic leakage was 2.99％ in the observation group,which was 11.94％in the control group,the difference was statistically significant (χ2 =3.890,P=0.049).Conclusion In the case of no difference in preoperative and postoperative perioperative management ,the left colonic artery and the anal canal were retained in the rectal cancer without affecting the lymph node dissection in No.253 group,but it could provide sufficient blood for the anastomosis ,to reduce the risk factor of the occurrence of anastomotic leakage .
RESUMEN
Objective To construct and identify the recombinant vector pcDNA3. 1 (-) B/myc-BRMS 1 carrying breast-cancer metastasis suppressor 1 (BRMS 1) which can express in eukaryote cells and which will provide the basis for further researching the mechanisms of metastasis suppression and working on cancer metastasis gene ther-apy. Methods To isolate total RNA from MCF - 7 cells and design a pair of primers, and coding sequence of aRMS 1 cDNA were amplified from human breast cancer cells MCF -7 by reverse transcription-polymerase chain reaction (RT-PCR). Then the product was inserted to the PcDNA3. 1/myc-His (-) B plasmid. The recombined pcDNA3. 1 (-)B/myc-BRMS1 was identified by gene sequence analysis,then recombinants was transfected into HEK-293 cells and was identified by Western blot. Results The recombinant of pcDNA3.1 (-) B/myc-BRMS1 was structurally confirmed by analysis of sequencing. The inserted fragment in the vector was in the right direction and its sequence was structurally confirmed to be consistent with CDS sequence of human BRMSI cDNA that of the published data. GenBank, [AF159141]. The recombinants was transfected into HEK-293 cells ,then the cells expressed protein tagged c-myc identified by Western blot indicated it can express in eukaryote cells. Conclusion cDNA of human BRMS1 can be successfully cloned and inserted into Eukaryote-expression vector. The newly constructed vector may serve as the potential tool to conduct further comprehensive experiments in future on BRMS1 function and on gene therapy.