Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
J Biosci ; 2003 Feb; 28(1): 51-5
Artículo en Inglés | IMSEAR | ID: sea-110999

RESUMEN

Fly ash was used as a model for ambient particulate matter which is under suspicion to cause adverse pulmonary health effects. The fly ash was pre-sized and contained only particles < 20 microm including an ultrafine fraction (< 100 nm) that contributed 31% to the particle number. In our study, we investigated the influence of fly ash on the promotion of early inflammatory reactions like the formation of reactive oxygen species (ROS) in rat lung epithelial cells (RLE-6TN). Furthermore, we determined the formation of nitric oxide (NO). The cells show a clear dose-response relationship concerning the formation of ROS with regard to the mass of particles applied. Lipopolysaccharide (LPS) added as a co-stimulus did not increase the formation of ROS induced by fly ash. Furthermore, in LPS (0.1 microg/ml) and tumour necrosis factor-alpha (TNF-alpha; 1 ng/ml) pre-treated cells no increase in reactive oxygen species comparable to fly ash alone is observable. In presence of the metal chelator, desferrioxamine (DFO), ROS formation can be significantly reduced. Neither fly ash nor LPS induced a significant NO release in RLE-6TN cells.


Asunto(s)
Contaminantes Atmosféricos/farmacología , Animales , Carbono/farmacología , Línea Celular , Transformación Celular Viral , Quelantes/farmacología , Medios de Cultivo/química , Deferoxamina/farmacología , Relación Dosis-Respuesta a Droga , Células Epiteliales/efectos de los fármacos , Lipopolisacáridos/farmacología , Pulmón/citología , Óxido Nítrico/biosíntesis , Nitritos/análisis , Tamaño de la Partícula , Material Particulado , Ratas , Especies Reactivas de Oxígeno/metabolismo , Acetato de Tetradecanoilforbol/farmacología , Factor de Necrosis Tumoral alfa/farmacología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA