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Journal of Gynecologic Oncology ; : 328-333, 2014.
Artículo en Inglés | WPRIM | ID: wpr-202217

RESUMEN

OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.


Asunto(s)
Animales , Femenino , Hormona Antimülleriana/sangre , Antineoplásicos/toxicidad , Biomarcadores/sangre , Cisplatino/toxicidad , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos/métodos , Preservación de la Fertilidad/métodos , Factor Estimulante de Colonias de Granulocitos/uso terapéutico , Folículo Ovárico/efectos de los fármacos , Insuficiencia Ovárica Primaria/sangre , Ratas Sprague-Dawley
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