RESUMEN
Objective:To determinate the concentration of Cisatracurium in the plasma of an rabbit via a proposed HPLC method. Methods:A C18 column was used,the mobile phase was made up of 50mmol/L dipotassium hydrogen phosphate(pH=3.0) and acetonitrile(50∶50),and examination and emission wavelengths are 200nm and 320nm,respectively. Results:the linear range was 25~2 000ng/ml,the limit of detection(LOD) is 25ng/ml, the intra- and inter-precisions(RSD) were lower than2.56% and 3.68%,respectively. Besides,the recovery of extraction was 85.2%~86.1%(n=5) and the recovery of addition was 93.4%~98.8%. Conclusion:The proposed method proved to be accurate and repetitive.
RESUMEN
OBJECTIVE:To determine the contents of betahistine hydrochloride and the related substances by HPLC.METHODS:The chromatographic separation was performed on Eclipse XDB-C18 column with column temperature at 25 ℃.The mobile phase consisted of water(sodium heptanesulfonate+10 mL triethylamine+810 mL water,with the pH adjusted to 3.0 with phosphoric acid) and methanol(82:18) at a flow rate of 0.8 mL?min-1.The detection wavelength was 261 nm and the sample size was 50 ?L.RESULTS:The linear range of betahistine hydrochloride was 10～80 ?g?mL-1(r=0.999 3).The lowest detection limit was 2.84 ?g?mL-1 and the lowest quantitative limit was 3.01 ?g?mL-1.The average recovery was 99.8%(RSD=0.3%).The average content for the related substances in 3 batches of samples was 0.13%.CONCLUSION:The method is simple,rapid,accurate and reliable,and suitable for the quality control of betahistine hydrochloride.