Gene Sequencing Analysis on Vibrio Cholera O_(139) Strains TcpA of Pingxiang Area / 中国医师杂志

Objective To investigate the TcpA gene sequence and analyze the characteristics of O 139 epidemic strains.Methods PCR was performed to detect the O 139 VC in the bacteria sample.The positive sample was sequenced and analysed.Results PCR products of one strain in the positive sample were sequenced.The gene sequences showed 100% nucleotide homology to O 139 strains adn Eltor strains 96.0%.Conclusion The present study indicates that O 139 VC and O1EVC in Pingxing area are genetically nearest in genetic relation.The study has significance in the diagnose and treatment of Vibrio cholerae in Pingxiang area.