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Introduction@#The aim of this research project is to elucidate the crosstalk of innate and adaptive immune reactions against the DNA containing bacteria.@*@#This study held in the Core laboratory, Science Technology Center, Mongolian National University of Medical Sciences (MNUMS). Murine aortal endothelial cells, END-D cultured and the cell viability checked by MTT assay. In addition, the NO production, protein and gene expression studied by Griess Reagent assay, R.T-PCR and immunoblotting, respectively. @*Results@#0.1µM, 1µM and 10µM of TLR9 ligand exhibited no cytotoxic action against the cells by MTT assay. IFN-ү alone induced NO production in END-D cells. In the other hand, TLR9 ligand at 0.1µM, 1µM and 10µM up-regulated IFN-ү induced NO production in dose dependent manner. RTPCR results exhibit that TLR9 ligand up regulates iNOS mRNA. Immunoblotting analysis showed the enhanced iNOS protein expression and phosphorylation of STAT1 in cells pre-treated with TLR9 ligand.@*Discussion@#We have demonstrated CpG DNA, TLR9 ligand, up-regulates IFN-ү induced NO via enhanced IFN-ү signaling. The result of Western Blotting and RT-PCR support the up-regulation of NO. CpG DNA can be used as agent against virus and bacteria. Further research need to be conducted. @*Conclusion@#TLR9 ligand, CpG DNA up-regulates IFN-ү induced NO production in time and dose dependent manner. TLR9 ligand augments the expression of iNOS mRNA and STAT1 phosphorylation in response to IFN-ү.
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@#BACKGROUND: Toll like receptors (TLRs) are a class of proteins that key role in the innate immune system. TLR7 is expressed on monocytes, macrophages and dendritic cells, T cell, B cell and eosinophiles. TLR7, originally identified as recognizing imidaquinoline, loxibrine, broprimine and ssRNA, ssRNA viruses such as vesicular stomatitis virus, influenza A virus and human immunodefiency virus. It is known that virus ssRNA affects signaling molecule of IFN-y. Objective: To determine gene and protein activation of IFN-y signal transduction by TLR7 ligand in the endothelial cells. MATERIAL: In study we used mouse aortic linear endothelial cell which is cultured (END-D) in 5% heat- inactivated fetal calf serum (FCS), medium (DMEM) containing antibiotic mix(penicillin G, streptomycin, amphotericin B) at 37°C (5% CO2). Endothelial cells treated with synthetic IFN-γ and imiquimodligands, then the NO (nitric oxide) concentration in the supernatant is determined by Griess reagent. Endothelial cells are cultured in 6 well cell culture plate and in each well 2*104cells are expected to be grown for 24 hours of culture. Then, the cells are treated with synthetic IFN-γ and имиквимод ligand for 6 hours and the NO signaling gene activation iNOS mRNA expression which is induced by IFN-γ is determined by RT-qPCR. Endothelial cells are cultured in 12 well cell culture plate and in each well 2*104 cells are expected to be grown for 18 hours of culture. Then, the cells are treated with synthetic IFN-γ and imiquimodligands for 24 hours and the NO signaling protein iNOS expression which is induced by IFN-γ is determined by western blotting. The experiment was conducted as representation mean of at least three test results. The difference between statistical probabilities is determined by the “Students” t test. The p<0.01 value is assumed to be statistically different. RESULTS: TLR7 ligand imiquimodaugmented interferon gamma induced nitric oxide production TLR7 ligand imiquimodincreased interferon gamma induced iNOS mRNA gene expression. TLR7 ilgand imiquimodup-regulated interferon gamma induced iNOS protein expression. CONCLUSIONS: TLR7 ligand imiquimod augments IFN-γ signaling in the endothelial cells. This synergistic effect has revealed in the levels of gene and protein expression.
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@#BACKGROUND OF STUDY: According to WHO report estimation, 76.3 million people have been diagnosed with disorder related to alcohol use which has been root cause for over 60 types of disease in the world.1 Smoking causes death to4 million people every year, 11 people every day and 6 every minute. This number is estimated to double reaching 10 million by 2030.2 The study conducted by the ‘World Vision Mongolia’ international organization and the Anti-alcoholism and Drug Association of the Ministry of Justice show 76% of all teenagers has drunk alcohol to some extent. AIMS: To determine alcohol, tobacco and psychoactive drug use by school teenagers of Sukhbaatar district in Ulaanbaatar MATERIAL AND METHODOLOGY: The survey on inquiry of alcohol, tobacco and psychoactive drug use (ASSIST) was conducted among a total of randomly selected 800 (374 boys, 426 girls) students of 8-12th grade from 7 secondary schools of Sukhbaatar district. Statistical data was put into Microsoft office applications (excel, word) and processed by SPSS application with 95% of probability of information and 5.0% of trust level. RESULT OF STUDY: Out of all respondents 32% (256) use tobacco, 43.8% (350)alcoholic drinks, 2.6% (21)cannabis,1.1% (9) cocaine, 1.5% (12) amphetamine, 3%(24)volatile substance, 4.3% (34)sedative and tranquilizer drug, 0.6% (5)hallucinogenic drug, 0.5% (4) opioid, and 0.1% (1) uses other types of drug. Among the survey participants, there are a total of 214 teenagers (in duplicated number) who need short-term action and 2 teenagers who are smoker and need intensive treatment. CONCLUSION: Among total teenagers, the survey participants, 1 in 3 used tobacco, 1 in 2 alcoholic drinks and 1 in 7 used psychoactive drug. Male students smoke twice more than female students and there is no difference in use of alcoholic drink in both sexes. The alcohol and tobacco use among teenagers at the age of 12-18 who participated in the survey showed that they use more when they grow older. 1 in 4 students of all participants belongs to a group with average risk and needs intensive treatment.
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Stigmatization and discrimination against mental patients and their family members due to typical community misunderstanding about mental diseases are commonly occurred among population. Particularly, discrimination against alcoholic patients and stigmatization against theirfamily members are most common among Mongolian people, because prevalence of alcohol dependence problems is increased 2 times more in last decades. Our aim was to define current situation of stigmatization and discrimination against alcoholic patients. This survey has been conducted among total 90 inpatients with alcoholic problem, are have staying in National Center for Mental Health and Narcology Center. We conducted the study by descriptive and crossectional design.Totally 90 alcohol patients, aged 19-61 years were participated in our survey. 67 (74.4%) of them were male and 23(25.5%) were female and average age was 40.1±1.1. Level of job’s discriminationwas growingrelated to increasing the level of alcohol consumption (p<0.0016)and level of family’s stigma was growing related to increasing the level of alcohol dependence (p<0.0001). Most of patients (n=29), who are divorced from their couples were answering that main reason of their divorcing was alcohol problem and bad interfamily relationship.Furthermore, stigmatization and discrimination increasing due to increasing level of alcohol consumption (p<0.0001).Most of participants (60%; n=54) are answered, that stigma and discrimination against them were occurring from their family.