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OBJECTIVE: To explore the cognition status and intervene needs for prevention of work-related musculoskeletal disorders( WMSD) among greenhouse farmers. METHODS: By purpose sampling method,1 780 participants were surveyed by the Cognition Status and Intervene Needs for Prevention of WMSD Questionnaires. By convenience sampling and judgement sampling method,14 respondents were interviewed. RESULTS: 66. 0 %( 1 174 /1 780) of subjects surveyed thought that WMSD was one of the most serious occupational hazard. Only 20. 3 %( 362 /1 780) of subjects well understood the knowledge of preventing WMSD. 54. 7 %( 974 /1 780) of subjects considered that they will protect themselves according to requirement. A total of 96. 7 %( 1 721 /1 780) respondents were willing to attend training classes.With the improvement of culture level,the awareness rate of WMSD severity was decreased( P < 0. 01),the awareness rate of WMSD prevention knowledge was increased( P < 0. 05) and the more was willing to attend training( P < 0. 05).The awareness rate of WMSD severity in plant length of service ≥ 20. 0 years was higher than that of plant length of service < 20. 0 years( 68. 9 % vs 61. 5 %,P < 0. 05). The awareness rate of WMSD severity in WMSD patients was higher than that of non-WMSD person( 67. 2 % vs 61. 6 %,P < 0. 05). The most needs for the greenhouse farmers was health education intervention methods,power tools and protection of improvement and development. CONCLUSION: The knowledge of preventing musculoskeletal disorders is deficient among greenhouse farmers. Health education suitable for farmers 'lifestyle should be launched in greenhouse and it is essential to devise protective equipment.
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<p><b>OBJECTIVE</b>To investigate the influence of fluorescent protein expression on the proliferation of murine NIH3T3 cells, so as to provide a theoretical basis for cell tracing technology.</p><p><b>METHODS</b>NIH3T3 cells were cultured in vitro, and were randomly divided into control, pLEGFP-N1 (with transfection of pLEGFP-N1 retroviral vector), pEGFP-N1 (with transfection of pEGFP-N1 vector) and pDsRed2-C1 (with transfection of pDsRed2-C1 vector) groups. Then the cells were screened by G418 for 3 weeks. The changes in cell adhesive rate were observed and the population doubling times was determined by growth curve.</p><p><b>RESULTS</b>There was obvious fluorescent protein expression in the transfected NIH3T3 cells after G418 selection, and the highest percentage of labeled NIH3T3 cells was found in pLEGFP-N1 group. The population doubling time in pDsRed2-C1 (40.3+/-0.7 h) , PEGFP-N1 (39.6 +/- 0.6 h) and pLEGFP-N1 (36.5 +/- 0.7 h) groups was evidently longer than that in control (27.9 +/- 0.6 h, P < 0.01), with high adhesive rate in each group.</p><p><b>CONCLUSION</b>The expression of fluorescent protein exhibited some inhibitory effect on the proliferation of NIH3T3 cells in vitro. Since the inhibitory effect by retroviral vector was weaker compared with eukaryotic vector, it should be the first choice for fluorescent protein labeling during cell transplantation.</p>