Proteomic Analysis of Colonic Mucosal Tissue from Tuberculous and Ulcerative Colitis Patients

Changes in the expression profiles of specific proteins leads to serious human diseases, including colitis. The proteomic changes related to colitis and the differential expression between tuberculous (TC) and ulcerative colitis (UC) in colon tissue from colitis patients has not been defined. We therefore performed a proteomic analysis of human TC and UC mucosal tissue. Total protein was obtained from the colon mucosal tissue of normal, TC, and UC patients, and resolved by 2-dimensional electrophoresis (2-DE). The results were analyzed with PDQuest using silver staining. We used matrix-assisted laser desorption ionization time-of-flight/time-of-flight spectrometry (MALDI TOF/TOF) to identify proteins differentially expressed in TC and UC. Of the over 1,000 proteins isolated, three in TC tissue and two in UC tissue displayed altered expression when compared to normal tissue. Moreover, two proteins were differentially expressed in a comparative analysis between TC and UC. These were identified as mutant beta-actin, alpha-enolase and Charcot-Leyden crystal protein. In particular, the expression of alpha-enolase was significantly greater in TC compared with normal tissue, but decreased in comparison to UC, implying that alpha-enolase may represent a biomarker for differential diagnosis of TC and UC. This study therefore provides a valuable resource for the molecular and diagnostic analysis of human colitis.

Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells in PGA Polymeric Scaffolds / 대한정형외과연구학회지

PURPOSE: To characterize the chondrogenic potential of human mesenchymal stem cells (MSCs) in porous polymeric scaffolds by poly (glycolic acid) (PGA) as three-dimensional constructs to facilitate chondrogenic differentiation. METHODS: Human MSCs were isolated by percoll gradient method and adherent cell cultures were obtained. Isolated MSCs were characterized with CD 34 and Sca-1 antibodies using flow cytometry. MSCs were seeded in the PGA polymeric scaffolds for 28 days in a specialized defined medium. The control group was examined without the specialized defined medium. The chondrogenesis of MSCs-seeded polymer scaffolds culture was assessed by histology, RT-PCR and 35S-sulfate incorporation. RESULTS: Flow cytometry result showed that CD 34 was negative and Sca-1 was 93+/-10% positive. By the histological analysis from Safranin-O staining, it was confirmed that the chondrogenic differentiated human MSCs expressed chondrocyte-like morphologies. We also observed that type II collagen was expressed by RT-PCR. The degree of proteoglycan synthesis was higher in the experimental group than the control group. CONCLUSION: We have demonstrated that the biodegradable porous polymeric scaffolds and the specialized defined medium is able to provide three-dimensional constructs for inducing chondrogenic differentiation of human MSCs.

Anterior Fixation Techniques on Unstable Pelvic Ring Injury / 대한정형외과학회잡지

PURPOSE: We reviewed the clinical result of two techniques of anterior fixation method for unstable pelvic ring injuries, which are external and internal fixation. MATERIALS AND METHODS: A retrospective clinical analysis was performed on 33 cases which had been operated from September 1998 to February 2003.Mean follow-up period is 29 months (range, 12-54 months).Previous to anterior fixation percutaneous iliosacral screw fixation was applied on iliosacral joint in Type C injury. After then plate and screw fixation were done in 17 cases and external fixation in 16 cases as an anterior fixation method. The patient' functional outcome was evaluated by Postel score and comparison between two groups was performed. RESULTS: In external fixator group the complaints are long duration of application of external fixator, excessive scar formation due to pin tract infection, pain and discomfort (Postel score 9.9;range, 8-11) due to malunion. In internal fixation group all of them showed more than good result and satisfactory to the postoperative condition and clinically (Postel score 11.6;range, 10-12). CONCLUSION: An open reduction and internal fixation technique is better than external fixation for the treatment of injured anterior pelvic ring in unstable pelvis fracture.