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Chinese Journal of Experimental and Clinical Virology ; (6): 297-302, 2019.
Artículo en Chino | WPRIM | ID: wpr-804831

RESUMEN

Objective@#To analyze the genetic characteristics of norovirus(NoV) in Jilin province from 2014 to 2016.@*Methods@#Stool samples from children under 5 years of age with diarrhea were collected from Changchun Children′s Hospital. Samples were amplified by RT-PCR. The polymerase and capsid gene of the positive specimens were amplified.Sequences were analyzed by homologous comparison and phylogenetic analysis.@*Results@#A total of 1 335 samples were detected, 177 were positive for NoV, and positive rate was 13.26%. The positive rate was 11.83% in 2014, 12.53% in 2015 and 15.75% in 2016. Genotyping of polymerase region revealed GII.P12 types dominated in 2014 and GII.Pe types dominated in 2015 and 2016. Genotyping of polymerase region revealed that GII.3 types dominated in 2014 and GII.4_Sydney2012 types dominated in 2015 and 2016. The types of both regions included GII.P17/GII.17, GII.Pe/GII.4_Sydney2012, GII.P12/GII.3, GII.Pe/GII.3、GII.P16/GII.2 and GII.P7/GII.6.@*Conclusions@#Genotypes of NoV infection are diverse in Jilin province. Most of them are recombinant strains. The main epidemic strains changed from GII.P12/GII.3 in 2014 to GII.Pe/GII.4_Sydney2012 in 2015 and 2016.

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