Study on the active constituents and molecular mechanism of Calyx seu Fructus Physalis in treating lung cancer based on network pharmacology / 国际中医中药杂志

Objective:With network pharmacology, this paper aims to explore the potential active constituents, related targets and signal pathways to elucidate the mechanism of Calyx seu Fructus Physalis in the treatment of lung cancer. Methods:By searching for Pharmacology Database of Traditional Chinese Medicine Systems and Analysis Platform (TCMSP) to obtain the active constituents of Calyx seu Fructus Physalis, and predict the target genes of active constituents by using SWISS and SEA. By collecting the corresponding targets approved by FDA and search for different database, including Therapeutic Target Database (TTD), Database of Gene-disease Associations (DisGeNET) and Online Mendelian Inheritance in OMIM to build a database of lung cancer related target genes; To get the target genes of herb-disease proteins by the intersection of the two databases and display the results through the network software of Cytoscape 3.7.2. Then to use the Database of Annotation, Visualization and Integrated Discovery (DAVID) to conduct key enrichment analysis of Kyoto Gene and Genome Encyclopedia (KEGG) pathway enrichment analysis. Combining related literature to analize the active ingredients and mechanism of Calyx seu Fructus Physalis in the prevention and treatment of lung cancer. Results:It was found that 11 active constituents of Calyx seu Fructus Physalis could play the role of anti-lung cancer by regulating 19 lung cancer related targets such as EGFR, ESR1, MDM2, MMP2 and MET. There were 15 signal pathways involved Proteoglycans in cancer, MicroRNAs in cancer, Pathways in cancer, Transcriptional misregulation in cancer, PI3K-Akt signaling pathway and other key signal pathways. Conclusion:The results show that the active constituents of anti-lung cancer effect of Calyx seu Fructus Physalis may be Oleic acid, Cycloartenol, Obturator, Graminesterol, β-sitosterol, etc. The mechanism of action may be related to multiple cancer-related signaling pathways such as Proteoglycans pathway, Transcriptional misregulation pathway, and PI3K-Akt signaling pathway.

Virtual screening the active ingredient and mechanism of Shenmai injection in treatment of novel coronavirus pneumonia based on network pharmacology and high throughput molecular docking / 国际中医中药杂志

Objective:To virtual screen the active ingredient of Shenmai injection in treatment of Novel Coronavirus Pneumonia and discuss the potential mechanism based on network pharmacology and high throughput molecular docking. Methods:Based on network pharmacology and high-throughput molecular docking technology, the compounds and predicted targets of Shenmai injection were retrieved from TCMSP, BATMAN-TCM and Targetnet databases, and the composition target map was constructed. The genes related to coronavirus pneumonia were retrieved from OMIM and GeneCards databases, and the PPI network between target genes was constructed by searching the common parts of target genes; David 6.8 was used to analyze gene function and pathway enrichment, and PDB database was used to obtain protein crystal structure, and Autodock Vina and python scripts were used for high-throughput molecular docking. Results:A total of 27 compounds and 224 target genes were obtained. 15 core components and 15 core targets for the treatment of coronavirus pneumonia were identified: CASP3, NOS2, PARP1, CASP8, NOS3, BCL2, ADA, OPRM1, TGFB1, TLR9, ACHE, SLC29A1, BAX, ADK, and PNP. The enrichment analysis showed that the core targets acted on the signaling pathways such as Tuberculosis, Pathways in cancer, Hepatitis B and Apoptosis. The better components of Novel Coronavirus Pneumonia related targets were diosgenin, stigmasterol, beta-sitosterol and ginsenoside Rh1_qt obtained by virtual screening.Conclusion:This study screened out the active ingredient and tarket of Shenmai Injection in treatment of Novel Coronavirus Pneumonia. It laid a foundation for the further clinical application of Shenmai injection and development of novel coronavirus pneumonia drugs.

Effects of Functional Point Injection on Upper Limbs Disorder in Cerebral Palsy Infants / 中国康复理论与实践

@# ObjectiveTo investigate the effects of functional point injection on upper limbs disorder in cerebral palsy infants. Methods120 cases were divided into 2 groups and each with 60 cases. The treatment group was given rehabilitation and functional point injection (injecting VitB1>, VitB12 in the acu-points selected at the disorder muscles) and the control group was given rehabilitation alone. All the cases were assessed with Peabody Developmental Motor Scale Fine Motor (PDMS-FM) and Modified Ashworth Scale (MAS). ResultsThe scores of both PDMS-FM and MAS improved in the treatment group compared with the control group (P<0.05). ConclusionFunctional point injection can improve the recovery of upper limbs disorder in cerebral palsy infants.

Misdiagnosis, Missed Diagnosis and Excesseive Diagnosis Related to Cerebral Palsy: 389 Case Report / 中国康复理论与实践

@#ObjectiveTo explore the state of misdiagnosis, missed diagnosis and excesseive diagnosis related to cerebral palsy(CP).Methods389 cases were retrospectively analyzed who were misdiagnosed, missed or escessively diagnosed related to CP as the first diagnosis in the inpatient and outpatient department from July 1999 to March 2010.ResultsAmong 389 cases, 156 cases were missed or misdiagnosed as nutritional disease, and 118 cases of other diseases were misdiagnosed as CP, while 115 cases who were normal children was excessively diagnosed as CP. The false diagnosis had relativity with children's age: doctors are more prone to make misdiagonsis when the children's age are smaller, while 293 cases were misdiagnosed before 12 months old (75.3%); 102 cases (65.4%) were misdiagnosed or missed as other diseases before September, 2004, while 87 cases (75.7%) were excessively diagnosed as CP after September, 2004. Frequency of misdiagnosis as CP reduced from 2004, and the proportion dropped from 55.9% to 44.1%.ConclusionIt is very important to master the diagnostic standard of CP. Both sides of the question are important, one side is to make early diagnosis and early treatment in order to achieve the best effect of rehabilitation, and the other side is to prevent misdiagnosis and excessive diagnosis which woud delay illness, or waste medical resources, increase the financial and psychological burden.

Clinical evaluation on effects of functional muscular equal pressure zone on complete denture / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the clinical effect of the functional muscular equal pressure zone on complete denture.</p><p><b>METHODS</b>Artificial teeth were arranged within the functional muscular equal pressure zone. With this technique 35 complete dentures were made to rehabilitate 35 edentulous patients whose alveolar bones were moderately or seriously absorbed. After three months' following up, the satisfaction of these patients were investigate.</p><p><b>RESULTS</b>After prosthesis treatment, 35 patients enunciated well and the aesthetic effect was favorable. 89 percent of these patients satisfied the retention and the stability of the maxillary denture, and 78 percent satisfied the retention and the stability of the mandibular denture. 79.5 percent of these patients satisfied the mastication efficiency.</p><p><b>CONCLUSION</b>With the technique, the complete denture is fairly stable in patient's mouth. The technique can enhance the denture's retention and improve the patient's mastication efficiency, especially to these whose alveolar bones are moderately or seriously absorbed. Clinically the technique is of high practical value to these edentulous patients who are difficult to be rehabilitated.</p>

Laboratory study on abutment movement of the combined clasp, the extension clasp and the telescopic crown / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the influence of three kinds of retainers on the abutment movement of removable partial dentures (RPDs) for restoring the dentition with unilateral distal-extended tooth missing.</p><p><b>METHODS</b>The combined clasp, the extension clasp and the telescopic crown were designed to retain RPDs. When food chip was masticated between dentitions, the displacement of abutments was measured by a high-sensitive-laser-transferring-detector, and then the data was statistically analyzed.</p><p><b>RESULTS</b>All of abutments showed the inclination, torsion and vertical translocation. Of them, abutments with telescopic crowns had the lowest horizontal torsion (P < 0.01) and the highest vertical translocation (P < 0.01).</p><p><b>CONCLUSIONS</b>Compared with the combined clasp and the extension clasp, the telescopic crowns enable abutments to bear the load more axial and the stress to be distributed more even, so the design of the telescopic crown may be considered more favourable for the health of abutments.</p>

Effects of finish line curvature on fitness of metal ceramic crowns / 实用口腔医学杂志

Objective:To investigate the effects of abutment finish line curvature on fitness of metal ceramic crowns.Methods:Three types of finish line curvatures' abutments were prepared (1 mm-curvature, 3 mm-curvature and 5 mm-curvature). For each type of abutment, ten metal ceramic crowns were fabricated (five for facial-veneered type, five for full-veneered type). Then the fitness of the crowns were evaluated. Results:In as-cast group, both for facial-veneered type and full-veneered type, the greater the finish line curvature was, the larger gaps the mesial and distal margins of copings exhibited. Compared with labial and lingual margins, the mesial and distal margins of copings for 5 mm-curvature crowns in full-veneered type showed significantly larger gaps (respectively 35?8 micrometers and 36?8 micrometers) (P

The influence of different inclination of cusp on the stress of an implant supported denture / 实用口腔医学杂志

Objective: To analyze the influence of different inclination of cusp on the stress of an implant supported denture. Methods: A spiral CT was used to scan a corpus' head.The model of mandible,teeth and implant was established by ANSYS 6.1 software. The superstructure of the denture had three different inclination of cusp.The stress distribution of the models under loading was analyzed. Results: When bearing non central loading, the maximum equivalent stress (max EQV) of the abutment was situated at the first screw of the abutment.When bearing central loading, the maximum equivalent stress(max EQV) of the abutment was situated at the cervix. Under all loading, the max EQV of the implant was situated at the cervix, the max EQV of mandible was situated at the upper cortical layer around the cervix of the implant. Higher inclination of cusp and non central loading lead to higher stress. Conclusion: The location of the max EQV of the abutment is consistent with the location of the abutment fracture; lower inclination of cusp and flatsurface teeth are benefit to the stress distribution of the implantation.

Experiment study on abutment displacement of bilateral distal-extension dentures with various retainers / 实用口腔医学杂志

Objective: To study the influence on the displacement of abutment with various retainer designs for bilateral distal-extension removable partial dentures(RPDs). Methods: Combined clasp, extension clasp and telescopic crown were used to retain RPDs. When food chip was masticated between the upper and lower dentition on one side, the displacement of abutment on both sides was measured by a high sensitive laser traneferring-detector and the data were statistically analyzed. Results: Inclination, torsion and vertical translocation of all abutment teeth were observed. Significant statistical difference ( P