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Braz. j. med. biol. res ; 45(3): 264-272, Mar. 2012. ilus, tab
Artículo en Inglés | LILACS | ID: lil-618056

RESUMEN

YKL-40 has been identified as a growth factor in connective tissue cells and also a migration factor in vascular smooth muscle cells. To a large extent, the increase of serum YKL-40 is attributed to liver fibrosis and asthma. However, the relationship of the expression and clinical/prognostic significance of YKL-40 to the splenomegaly of patients with portal hypertension is unclear. In the present study, the expression of YKL-40 was studied by immunohistochemistry in 48 splenomegaly tissue samples from patients with portal hypertension and in 14 normal spleen specimens. All specimens were quickly stored at -80°C after resection. Primary antibodies YKL-40 (1:150 dilution, rabbit polyclonal IgG) and MMP-9 (1:200 dilution, rabbit monoclonal IgG) and antirabbit immunoglobulins (HRP K4010) were used in this study. The relationship of clinicopathologic features with YKL-40 is presented. The expression of YKL-40 indicated by increased immunochemical reactivity was significantly up-regulated in splenomegaly tissues compared to normal spleen tissues. Overexpression of YKL-40 was found in 68.8 percent of splenomegaly tissues and was significantly associated with Child-Pugh classification (P = 0.000), free portal pressure (correlation coefficient = 0.499, P < 0.01) and spleen fibrosis (correlation coefficient = 0.857, P < 0.01). Further study showed a significant correlation between YKL-40 and MMP-9 (correlation coefficient = -0.839, P < 0.01), indicating that YKL-40 might be an accelerator of spleen tissue remodeling by inhibiting the expression of MMP-9. In conclusion, YKL-40 is an important factor involved in the remodeling of spleen tissue of portal hypertension patients and can be used as a therapeutic target for splenomegaly.


Asunto(s)
Adulto , Anciano , Animales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Conejos , Adulto Joven , Adipoquinas/metabolismo , Hipertensión Portal/metabolismo , Lectinas/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Bazo/metabolismo , Esplenomegalia/metabolismo , Biomarcadores/metabolismo , Estudios de Casos y Controles , Hipertensión Portal/complicaciones , Esplenomegalia/etiología
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