Determination of kaempferol and quercetin in xindakang tablet by beta-cyclodextrin modified micellar electrokinetic capillary chromatography

A method was proposed to determine kaempferol and quercetin in Hippophae rhamnoides L medicinal preparation xindakang tablet by beta-cyclodextrin modified micellar electrokinetic capillary chromatography. Under the optimized conditions: buffer solution of 20 mmol/L Na[2]B[4]O[7]-KH[2]PO[4] [pH 9.0]-20mmol/L SDS-6mmol/L beta-CD-5%[v/v] MeCN, applied voltage of 16 kV and injection time of 8s, the two analytes were separated well within 10 minutes. The calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/mL range for kaempferol and quercetin, respectively. The reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. The detection limits based on three times noise were 0.010 mg/mL and 0.008 mg/mL for kaempferol and quercetin, respectively. The developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.

Studies on Total Flavonoids from Root,Stem and Leaf of Glabrous Sarcandra(Sarcandra glabra) / 中草药

Total flavonoids from root,stem and leaf of sarcandra glabra(Thunb.)Nakai were extracted and deter　mined. Results showed that total flavonoids existed mainly in leaf of S. glabra(Thunb.)Nakai,and extraction time should be more than 3h