RESUMEN
Calli have been induced on an axenic hypocotyl explants excisized from germinated seeds of Solarium nigrum under tissue culture techniques using MS-medium supplemented with 0.3 mg/L NAA and 2.0 mg/L kinetin. Calli were then removed and subcultured on medium either without salt [control] or provided with either of NaCl concentrations [50, 100, 150 and 200 mM] then kept growing at 28degree under low light intensity for 8 weeks. During the first 4 weeks, the salt treated calli grow more slowly than the control and most of them [70 percent] specially at the higher salt concentrations [150, 200 mM] became necrotic. Those that survived and adapted to salt medium subsequently grew more quickly than controls especially at lower salt concentrations [50, 100 mM]. Calli collected and subjected immediately for the lipid analysis. The results obtained showed with the slight increase of NaCl [50, 100 mM], no significant changes detected in neutral or phospholipids, but at the higher NaCl concentrations [150, 200 mM] most of the individual classes of neutral, phospho and the total lipids have shown a significant decrease. Few lipid classes have an increase at the higher salinity levels [150, 200 mM] e.g., diacyl glycerol and sterol from neutral lipids and phosphatidic acid from the phospholipids