RESUMEN
To address the question if an altered oral microbiota is associated with atherosclerosis. Twenty patients suffering from atherosclerosis and 10 controls were recruited. Clinical oral, medical and laboratory investigations were performed. Oral bacteria were collected and 16S rDNA was sequenced following Single strand conformation polymorphism.(SSCP) Probing pocket depths in patients were significantly elevated. The oral microbiota of patients and controls were dominated by Fusobacterium (16%/17%), Streptococcus (21%/14%), Prevotella (10%/12%), Enterococcus (12%/12%), Porphyromonas (8%/7%), TM7 (0%/7%) and Veillonella (6%/7%). Differences in diversity were not significant between groups. The pathology of atherosclerosis may not be related to significant qualitative changes of the oral microbiota.
Asunto(s)
Grupos Control , Placa Dental/análisis , Placa Dental/microbiología , ADN Bacteriano/análisis , Fusobacterium/análisis , Fusobacterium/aislamiento & purificación , Humanos , Metagenómica/métodos , Boca/microbiología , Pacientes , Prevotella/aislamiento & purificación , ARN Ribosómico 16S/análisis , Streptococcus/análisis , Streptococcus/aislamiento & purificación , Veillonella/análisis , Veillonella/aislamiento & purificaciónRESUMEN
Atualmente vários protocolos comerciais e näo comerciais para preparaçäo de plasmídeos estäo disponíveis. Contudo, os kits säo caros e muitos dos protocolos contêm substâncias químicas tóxicas. Apresentamos neste trabalho um novo, otimizado e portanto muito vantajoso protocolo para preparação de plasmídios usando n-butanol. A preparaçäo pode ser efetuada rapidamente, sem adiçäo de substâncias químicas tóxicas e a um custo total de aproximadamente um centavo (americano) por preparaçäo.