RESUMEN
Statement of the Problem: Genetic polymorphisms can alter immunity response against pathogens, which in turn influence individuals' susceptibility to certain infections
Purpose: Our aim was to determine the association of Arg753Gln [rs5743708] and Arg677Trp [rs12191786] polymorphisms of toll like receptor-2 gene with the two clinical forms of apical periodontitis: acute apical abscess [AAA] and asymptomatic apical periodontitis [AAP]
Materials and Method: There were 50 patients with AAA as case group and 50 with AAP as control group. Genotyping was done using Tetra-ARMS [amplification refractory mutation system] PCR
Results: Heterozygous genotype of Arg677Trp polymorphism was associated with risk of AAA [OR=1.9, 95% CI: 0.7-5.5, p= 0.05]. Although statistically insignificant, Arg677Trp polymorphism promoted the risk of AAA in dominant model [OR=2.1, 95% CI: 0.7-5.9, p> 0.05]. The frequency of mutant allele [T] of Arg677Trp polymorphism was higher in AAA [14%] than AAP [7%] subjects [OR=1.7, 95% CI: 0.6-4.7]. For Arg753Gln polymorphism, wild homozygous [GG] represented the dominant genotype in both cases [96%] and controls [100%]. Variant allele [A] of Arg753Gln polymorphism was identified in 2% of AAA, while no individual represented with this allele in AAP subjects. Individuals with Arg753Gln; Arg677Trp [GG; TC] combination showed an elevated risk of AAA [OR=1.6, 95% CI: 0.5- 4.2, p> 0.05]
Conclusion: Arg677Trp polymorphism of TLR-2 rendered a higher risk for the development of abscesses in apical periodontitis. It is recommended to explore role of this polymorphism in other populations
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The aim of this study was to determine the prevalence and viral load of Epstein-Barr virus [EBV] and Human herpesvirus-6 [HHV-6] in different histopathologic grades of oral squamous cell carcinoma [OSCC]. Forty-five formalin-fixed paraffin-embedded tissue section of OSCC patients were analyzed by quantitative real-time polymerase chain reaction for detection of EBV and HHV-6. The mean age of the patients was 58.6 years, 69% of whom were female, and 31% were male. Overall, the positive rate for EBV and HHV-6 were 16.7% and 27.1%, respectively; and the mean viral load EBV was 27.9 x 10[3] and 38.5 x 10[3] for HHV-6. No correlation was demonstrated between the viral load of EBV DNA [P = 0.35] and HHV-6 [P = 0.38] at the different OSCC histopathologic grades. These findings neither lend support to the hypothesis that EBV and HHV-6 are directly involved in OSCC nor rule out the possibility that these viruses play an indirect role in carcinogenesis in this area
Asunto(s)
Humanos , Masculino , Femenino , Carcinoma de Células Escamosas , Herpesvirus Humano 6 , Infecciones por Roseolovirus , Herpesvirus Humano 4 , Infecciones por Virus de Epstein-BarrRESUMEN
Occurrence of leukemia in thalassemia major is a rare presentation. Here we report two cases of thalassemic patients, developing acute lymphoblastic leukemia. The genetic analysis revealed that, female and male patients were homozygous for IVSI-6 and IVSI-5, respectively. Two years ago the female patient presented by a high leukocyte count [154,000 micro L] and male one also presented by 80,000 WBC/micro L count 1 year ago. Microscopic examination of both patients revealed lymphoblasts that morphologically accommodate with ALL-L1 that were confirmed by photocytometry
RESUMEN
OBJECTIVE@#To determine the distribution of Duffy blood group genotypes in Balouch population as a major ethnic group that living in a sub-tropical area in south East of Iran.@*METHODS@#In this study, the Duffy blood group FY phenotypes were determined using indirect anti-globulin technique and also genotype by PCR-RFLP in 160 vivax malaria patients and 160 control individuals.@*RESULTS@#The results showed that the most common Duffy genotype was FYA/FYB (46.6%) followed by FYA/FYA (15.3%), FYA/FYO (14.4%), FYB/FYO (11.9%), FYB/FYB (10%) and FYO/FYO (1.9%). In case individuals, frequency of FYA, FYB and FYO alleles were 0.471, 0.431 and 0.097, respectively compaired to 0.444, 0.353 and 0.203, respectively in control (non-infected) group.@*CONCLUSIONS@#This data provide evidence that individuals with the FYA/FYB genotype have higher susceptibility to malaria and there are significant associations between Duffy blood group variants and susceptibility or resistance to vivax malaria.