Asunto(s)
Humanos , Prueba de Histocompatibilidad , Complejo Mayor de Histocompatibilidad/inmunología , Antígenos de Histocompatibilidad Clase II , Formación de Anticuerpos/fisiología , Complejo Mayor de Histocompatibilidad/genética , Polimorfismo de Longitud del Fragmento de Restricción , Pruebas Inmunológicas de Citotoxicidad/métodosRESUMEN
We measured total serum IgE in 14 patients with allergic diseases and 16 healthy subjects, using 3 commercial ELISA kits. The correlation of results among the 3 kits was analized using Passing and Bablock regression parameters. Results show that measurements of the different kits do not coincide. One kit shows differences using sera from allergic patients. It is concluded that it is not possible to determine exactly the amount of IgE using these kits, specially in subjects with elevated levels
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Inmunoglobulina E/análisis , Ensayo de Inmunoadsorción Enzimática , Hipersensibilidad Inmediata/inmunología , Estudios de Casos y ControlesRESUMEN
Pre S1 antigen was measured using an ELISA technique in patients with different forms of hepatitis B virus infection. It was detected in 10 of 19 patients with acute hepatitis B (53 percent), 12 of 15 chronic hepatitis B virus carriers (80 percent), 9 of 11 patients with chronic hepatitis b (82 percent) and 3 of 4 patients with hepatoma and positive markers of hepatitis B virus infection. Pre S1 remained positive beyond 150 days in two patients with acute hepatitis that evolved to chronicity. Among subjects with chronic hepatitis B that received interferon, pre S1 antigen negativized only in the patient that had a complete response. Pre S1 detection is an index of hepatitis B virus replication and its persistence determines chronicity. Its negativization after antiviral therapy should have a predictive value