RESUMEN
After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca2+ entry. 2-APB inhibition of sarcoplasmic reticulum Ca2+ release was excluded because this blocker did not affect serotonin force development in a Ca2+-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs.
Asunto(s)
Humanos , Compuestos de Boro/farmacología , Contracción Muscular/efectos de los fármacos , Músculo Liso/efectos de los fármacos , Músculo Liso/metabolismo , ARN Mensajero/genética , Arterias Umbilicales/efectos de los fármacos , Capacitancia Vascular/efectos de los fármacos , Western Blotting , Células Cultivadas , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/química , Canales de Calcio/genética , Canales de Calcio/metabolismo , Calcio/metabolismo , Agonistas de los Receptores Histamínicos/farmacología , Histamina/farmacología , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Músculo Liso/citología , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Retículo Sarcoplasmático/efectos de los fármacos , Retículo Sarcoplasmático/metabolismo , Agonistas de Receptores de Serotonina/farmacología , Serotonina/farmacología , Canales Catiónicos TRPC/genética , Canales Catiónicos TRPC/metabolismo , Arterias Umbilicales/citología , Arterias Umbilicales/metabolismoRESUMEN
El fumar tabaco durante el embarazo aumenta los riesgos en la salud materno-infanto juvenil provocando múltiples alteraciones atribuidas a la nicotina y al monóxido de carbono producidos por el cigarrillo.