Effect of food colouring metanil yellow on the liver and brain of albino rats

Forty two adult male albino rats were used in this study and divided into four groups. The first served as a control, the 2[nd], 3[rd] and [4th] were oral treated by metanilyellow for 3, 7 and 15 days. The results showed significant increase of hepatic glutathione peroxidase, lipid peroxidation, glutathione S-transferase, cytochrome P-450 and significant degrease of reduced glutathione content in all treated groups. Also, this study showed significant increase of noradrenaline and significant decrease of dopamine and serotonin in the brain of all treated groups. The present study showed histopathological changes of both liver and brain of albino rats

comparative-study on enzymatic changes in the serum and synovial fluid at early post-mortem period

In this study, postmortem blood and synovial fluid samples were taken from 50 cadavers, from Zeinhom's mortuary. Their medico-legal reports were revised thoroughly to obtain the precise time of death. Serum and synovial fluid samples were analyzed and the levels of the following enzymes were estimated. Alkaline phosphatase [Alk. Ph], Aspartate aminotransferase [AST] Alanine aminotransferse [ALT] and Lactate dehydrogenaze [LDH]. Postmortem serum and synovial fluid samples were grouped according to postmortem interval [the time of samples withdrawal in the first twelve hours ostmortem]. This study showed that the values of Alk. Ph. AST, ALT and LDH increased progressively until 12 hours P.M. It was observed that, there was no significant difference between the mean enzyme levels in the serum and synovial fluid. It is suggested that the mean enzyme levels in the synovial fluid can be used to predict their levels in the serum with much reliability. Moreover, synovial fluid can be easily obtained and does not generate cosmetic problems. It is concluded that synovial fluid analysis can provide useful information in autopsy cases in which suitable blood or urine specimens can not be obtained

Determination of MN blood groups

In this study, samples of blood were collected from two hundred femalevolunteers aging between 18 and 23 years old. Two methods for MN groupingwere done, the first was "tube technique method", while the second was "tilemethod". Also, factors which may affect grouping by the two methods includingtime of reading of the reaction and the dilution of antiserum were tried. Theresults of grouping by using both tube and tile methods indicated that thehighest percentage [46%] was for MN blood group, followed by M blood group[34%], while N blood group showed the lowest one [20%]. Tube methodapplication revealed that M antigen was more potent than N antigen, so itsreaction was stronger. It was also found that best results can be obtainedwhen the antisera used are fresh, of a dilution ranging between 1/32 up to1/64 and the best time of reading of the reaction is after lapse of 3 hoursbut can be read with less strength after 2 hours. When the tile method wasapplied, strong +ve results for M antigen was found, and the best time ofreading was after 30 seconds from adding corresponding RBCs. The bestsuitable dilution for the reaction was 1/32 up to 1/64 for MN grouping