Bioactivities of Alchemilla mollis, Alchemilla persica and Their Active Constituents

Abstract The aim of this study was to determine antimicrobial activities of Alchemilla mollis, Alchemilla persica as well as ellagic acid and miquelianin against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans by using microbroth dilution method and anti-inflammatory activity by using human red blood cell (HRBC) membrane stabilization method. Microbroth dilution method was used to determine the antimicrobial activities. Extracts possessed activity having MIC values of 2.5-5-10mg/ mL, compounds possessed activity having MIC values of 1.25-2.5-4-5mg/mL. A.mollis aerial parts displayed the highest anti-inflammatory activity (IC50=1.22±0.07mg/mL). Ellagic acid and miquelianin were also determined as anti-inflammatory agents with 0.57±0.01mg/mL and 1.23±0.02mg/mL IC50 values, respectively. Total phenolic content and tannin content of the A.mollis and A.persica were determined as 357.00±75.80mg, 282.50±28.70mg PGE/g plant material and 18.02%, 18.63% respectively according to the method described by European Pharmacopoeia. Ellagic acid, miquelianin and catechin were analyzed by HPLC. The highest catechin content was detected in A.persica roots (6.69±0.05g/100g plant material). A.mollis aerial parts contain higher miquelianin (0.39±0.02g/plant material) and ellagic acid (1.56±0.01g/ plant material) than A.persica.

In vitro Antimicrobial Activity of Various Mouth Rinses against Streptococcus mutans, Lactobacillus casei, L. acidophilus and Candida albicans.

Aim: The aim of this in vitro study was to determine the antimicrobial activities of commonly used mouth rinses against different oral microorganisms. Methodology: Six commercially available mouth rinses (Colgate Total, Colgate Plax, Colgate Pro-argin, Oral-B Pro-expert, Listerine and Oderol) were used in this study. The antimicrobial activities of mouth rinses against Streptococcus mutans ATCC 25175, Lactobacillus casei RSKK 900, L. acidophilus ATCC 11975 and Candida albicans ATCC 10231 were evaluated by disc diffusion and minimum inhibitory concentration tests. Klorhex® was used as positive control. Experiments were repeated twice. Results: Colgate Total, Colgate Plax, Oral B Pro-expert and Oderol showed good antimicrobial activity against test microorganisms in comparison with Colgate Pro-argin and Listerine. Conclusion: Chlorhexidine and cetylpyridinium chloride containing mouth rinses were found more active than other products tested. Main motive of mouth rinses is removing the microorganisms from the mouth although their effectiveness depends on the formulation, concentration, quantity of ingredients and duration of rinsing.