RESUMEN
One of the most economically important bacterial pathogens of plants and plant products is
Asunto(s)
Bacteriófagos/aislamiento & purificación , Agentes de Control Biológico/aislamiento & purificación , Dickeya chrysanthemi/crecimiento & desarrollo , Dickeya chrysanthemi/virología , Enfermedades de las Plantas/microbiología , Solanum tuberosum/microbiología , Secuencia de Bases , Bacteriófagos/clasificación , Agentes de Control Biológico/clasificación , ADN Bacteriano/genética , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Myoviridae/clasificación , Myoviridae/aislamiento & purificación , Dickeya chrysanthemi/efectos de los fármacos , Dickeya chrysanthemi/aislamiento & purificación , /genética , Análisis de Secuencia de ADN , Siphoviridae/clasificación , Siphoviridae/aislamiento & purificaciónRESUMEN
The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after fermentation and separation the cells, was lyophilized and the activity was measured. Total activity of this strain was 12 kU/g that shows its potential for industrial uses. Further study is required for purification of enzyme and calculation its specific activity. Immobilization is another approach should be considered.