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Annals of Clinical Microbiology ; : 20-23, 2016.
Artículo en Inglés | WPRIM | ID: wpr-151566

RESUMEN

In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.


Asunto(s)
Pared Celular , Mycobacterium tuberculosis , Mycobacterium , Ácidos Micólicos , ARN
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