Granulomatous inflammatory response in a case of typhoid fever

To present a case of culture-proven typhoid fever in which granulomas were demonstrated histologically in the ileum and mesenteric lymph nodes. A 47-year-old Pakistani man underwent emergency hemicolectomy for severe bleeding per rectum associated with diarrhea. Two large ulcers in the ileum, near the ileocolic junction, as well as mesenteric lymph nodes showed both necrotizing and non-necrotizing granulomas in addition to mixed inflammatory infiltrate on histology. Typhoid fever may be considered as one of the causes of the differential diagnoses of granulomatous inflammation of the small intestine

[Study of lytic phage and alkaline phosphatase on treatment of burn infections caused by Escherichia coli in mouse]

Bacteriophages are microorganisms that have been using bacterial hosts for propagation and life cycle. Some of them are called lytic bacteriophages, that lyse bacterial hosts after growth. These kinds of bacteriophages are used for treatment of bacterial infections and phage typing. Lytic bacteriophages have several advantages as a treatment against infections in contrast with antibiotics. Therapeutic effects of lytic phage isolated from natural habitates were studied against burn infection of Escherichia coli. Lytic bacteriophages were isolated from environmental resources using luria broth and overlay method. Then phages propagated using Escherichia coli as host, supernatant filtered, and after precipitation of polysaccharides used for treatment of Escherichia coli infections. For treatment experiments induced burn infections in laboratory mouse were used. Lytic bacteriophages were isolated frequently from human and sheep stools, and sewage. Phage titer isolated from sheep's stool was higher than other samples. X[2] analyses results indicate that there was significant difference in death incidence of studied groups. Based on statistical analysis using lytic bacteriophage for treatment of burn infection of Escherichia coli inhibited 80% of mouse from death. Results emphesis the potential of bacteriophages as potent antibacterial treatments