[Comparative study on 1D [VP1] region of foot and mouth disease virus [type A strain] among different isolates: Khorasan Razavi isolate and other Iranian and neighboring countries isolates]

Foot-and-mouth disease [FMD] is one of the most important virus disease in farm animals. Types O, A and Asial FMD virus have been endemic in Iran. In this study, samples from suspected livestock were analyzed by RT-PCR experiment. The number of 702 nucleotides determined at 1D- 2B region of type A strain isolated from Khorasan Razavi province sequenced and compared with that of other reported isolates type A from Iran and neighboring countries. The results show that field isolated type A has about 89% similarity with other reported isolates type A from Iran and neighboring countries. Furthermore, this virus shows the most similarity with A/IRN/1/87[Samuel. Phylogenitic analysis revealed that virus was closely related to A22-Iraq/99 and A/IRN/iso/105 that rest in the same lineage. The data showed high similarity between type A viruses involved in the Khorasan Razavi province and A/IRN/87v [vaccine strain]; so that it can be concluded that the vaccine can produce prophylactic antibody against this virus

Study on distribution of infectious hematopoietic necrosis [IHN] in five major provinces producing rainbowtrout [oncorhynchus mykiss] fry in Iran by indirect fluorescence antibody [IFAT] and nested -RT- PCR techniques

Distribution of infectious hematopoietic necrosis[IHN] was studied in five major provinces producing rainbow trout[Oncorhynchus mykiss]fry in Iran by indirect fluorescence antibody IFAT and nested-RT-PCR techniques. Also the effect of time duration was examined on some positive samples after 8 months post sampling. Samples of kidney, liver and spleen of rainbow trout fries collected from 27 trout farms were processed and examinated. Fourteen trout hatcher is located in all provinces were identified to be contaminated with IHNV tested by both techniques. The obtained results also showed that nested-RT-PCR is more sensitive than IFAT test particularly if samples are stored for a longer time

[Introduction of isolated salmonellae from human and animal, and the outlook to epidemiology of salmonellosis in the chaharmahal bakhtiary province in Iran]

The survey was conducted on 2800 cases of animal and animal samples which were referred to veterinary clinic of shahrekord [center of Chaharmahal Bakhtiary Province in Iran], and 591 cases of human suspicious to salmonellosis that referred to infectious section of Ayatolah Kashany Hospital of Shahrekord, during December 1990 to September 1992. All of the samples were cultured on special bacteriological media of salmonella. The isolated salmonellae from those samples, identified by serological method [Kuffman-White]. From 2175 perished poultry 658 [30.25%] Salmonellae were isolated that 97% of them, were S. enteritidis and 7% were S.typhimurium. From 375 aborted sheep and goat fetus, 7 [1.8%] Salmonella abortus ovis were isolated. From 45 cases of diarhetic cows and calves 7 [15.5%] salmonellae were isolated, that 71% of them S. enteritidis and 29% were S.typhimurium. From 20 case of human that suspicious to salmonellosis collected the bone marrow, 3 [15%] salmonellae were isolated which 66.61 [2 case] of them were S.typhi and 33.3 [one case] was S.enteritidis. From 429 case of human blood culture suspicious to salmonellosis 13 [3.0%] salmonellae were isolated that 61.5% of them were S.typhi and 23.1% S.paratyphi A and 15.4% were S.enteritidis. The result obtain in the survey showed that 89.5% of salmonellae were S.enteritidis. S.enteritidis is an important cause of enteritis and food poisoning in human and animal. Serological study in human population in Chaharmahal Bakhtiary in this survey showed that 1.55% of persons that referred to medical diagnostic laboratories in Chaharmahal Bakhtiary province were positive in vidal test that 9.16% of them were related to group A salmonellae, 18.3% to group B salmonellae and 72.5% to group D salmonellae