RESUMEN
Background: hydatidosis is one of the zoonotic diseases which affect animals and human beings at the larval stage of Echinococcusgranulosus, thereby playing a role in exacerbating the economic and health problems in Iran
Objectives: this study was conducted to investigate the strains of Echinococcusgranulosus isolated from cattle hydatid cysts, in Khorramabad, in the Lorestan Province, in the west of Iran
Methods: twenty-six isolates of hydatid cyst of cattle from lung [24 samples] and liver [2 sample] organs were collected from Golshan Slaughterhouse, in Khorramabad. All of the samples were transferred to the laboratory for morphometric characterization and molecular study. In morphological characterization, blade length of large [LBL] and small [SBL] hooks and the ratio of blade length to total length in large [LBL/LTL] and small [SBL/STL] hooks and total length of large [LTL] and small [STL] hooks were measured. In molecular study, a partial sequence of cytochrome oxidase 1 [CO1] with 440 bp in length was amplified applying primers J3 and J4.5. Genomic DNA sequencing was performed by Sanger's method
Results: the morphological results showed that there is no significant difference between isolated from cattle hydatid cyst and sensu stricto strain [p<0.05]. The results of molecular studies support the findings of morphological characterization. All sequences showed 100% identity with sensu stricto strain
Conclusions: the results from this study showed that sensu stricto strain [G1] is a causative agent of cattle hydatid cyst in Khorramabad. On the other hand, the cattle play a role in enabling the parasite to complete its cycle. Hence in order to execute a control program for minimizing the effects of this disease, the cattle should be considered as a source of infection for dogs. The results of this study could be helpful in designing such control program in the region