Protective effect of two anti-hepato toxic agents: silymarin and alpha-tocopherol against the subcellular toxicity induced by the hypolipidemic agent etofibrate

Etofibrate, a nicotinoyl derivative of clofibrate which belongs to the family of fibric acids. Both nicotinic acid and clofibrate are known for their hypolipidemic action and are used for the treatment of hyperlipoproteinemia. In the present study, the effect of three concentration levels of etofibrate on four rat liver lysosomal enzymes [Acid phosphatase, beta-Galactosidase, B, N-Acetyl glucosaminidase and b-Glucuronidase] for three incubation periods [30, 60 and 120 minutes] were studied in vitro, and were then compared with their combined effects together with a single therapeutic dose of each of two anti-hepato toxic agents. Silymarin and alpha-tocopherol [vitamin E] separately on the same enzymes for the same incubation periods. The results obtained showed that the three doses of etofibrate induced a marked releasing effect on the four lysosomal enzymes irrespect of the incubation time. Meanwhile, a considerable stabilization of these enzymes was observed when each of the two single doses for alpha-tocopherol and silymarin were used separately with etofibrate at the lowest concentration level

Effect of the hypolipidemic agent gemfibrozil and its combination with two anti-hepato toxic agents silymarin and alpha-tocopherol on rat liver lysosomal enzymes in vitro

Gemfibrozil, is a potent hypolipidemic agent used for the treatment of elevated levels of plasma triglycerides and very low density lipoprotein cholesterol [VLDL]. In this study, the effect of three sole graded concentrations of gemfibrozil on four rat liver lysosomal acid hydrolases [Acid phosphatase, beta-Galactosidase, b.N. Acetyl glucosaminidase and beta-Glucuronidase] were studied for three incubation periods [30, 60 and 120 minutes] in vitro. The effect of the same concentration levels of gemfibrozil, each combined with a single therapeutic dose of two anti-hepatotoxic agents: silymarin and alpha-tocopherol on the same lysosomal enzymes for the same incubation periods were also investigated. The results of this study showed that gemfibrozil displayed a highly significant releasing effect on the four lysosomal enzymes regardless of the dose or the incubation time, and that such effect presists even in the presence of the two membrane protecting agents: silymarin and alpha tocopherol