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To explore the efficacy and feasibility of interstitial chemotherapy using thermosensitive gel-coated ricin in hepatoma H22-bearing mice.
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Objective To evaluate the effectiveness and feasibility of riein-loaded thermosenaitive hydrogel in percutaneous intratumoral injection for treatment of hepatic carcinoma. Methods BALB/c-nu mice were inoculated subcutaneously in the right flank with human liver cancer cell line HepG2. A total of 32 male mice bearing subcutaneous hepatic carcinoma (7-10mm in diameter) were randomized into 4 groups. Each group of mice were administered percutaneous intratumoral injection of different agents as follows: salin, thermosensitive hydrogel,ricin, ricin-loaded thermosensitive hydrogel. The tumor volume was totally measured 3 times by high frequency ultrasound once a week. One mouse of each group was sacrificed to perform tumor histopathological examination on 7th day, 14th day, respectively, and survival time of the remaining mice was recorded. Results After intratumoral therapy,tumor volume in ricin group was smaller than that of saline group on 7th day, 14th day and 21st day( P<0.01 ),and mice got a life-prolonging rate of 36.8%. In ricin-loaded thermosensitive hydrogel group,obvious necrosis occurred in tumors treated since 5th day, and the tumor almost regressed on 21st day. Histopathological assay showed serious necrosis with a large number of leucocyte infiltration in tumor. All the remaining mice treated with riein-loaded thermosensitive hydrogel survived at the end of experiment sans tumor recurrence. Conclusions Percutaneous intratutnoral injection of ricin-loaded thermosensitive hydrogel could completely ablate hepatic carcinoma,which may provide an effective and feasible method for tumor ablation therapy.
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The expression of nm23 gene product/NDPK was studied in curatively resected human gastric carcinoma, using anti-NDPK polyclonal antibody. Of 115 patients tested, 59.1%(68/115) showed positive staining in the primary lesions, and 41.9%(36/86) were positive in the metastatic regional lymph nodes. A significantly higher positive staining rate was found in patients without regional nodal metastasis (86.2%, 25/29) than in those with nodal involvement (50.0%, 43/86; P
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ObjectiveTo evaluate the expression of RANKL′s protein for the diagnosis and assessment of tumor metastatic potential and prognosis in patients with gastric carcinoma.MethodsThe expression of RANKL was detected in 45 cases of gastric carcinoma, 26 cases of paracancerous mucosa and 10 cases of healthy control by using ABC immunohistochemical method. Data were tested by ? 2 test for statistical significance.ResultsPositive expression of RANKL protein was detected in 21 out of 45(46 7%) cases. The expression of RANKL significantly increased in tumors ≥5?cm in diameter, with serosa infiltrated, positive lymph node, and in tumors of stage Ⅱ,Ⅲ,Ⅳ( P