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Acta Pharmaceutica Sinica ; (12): 787-792, 2011.
Artículo en Chino | WPRIM | ID: wpr-233055

RESUMEN

FGF21 (fibroblast growth factor 21) is a recently described member of the FGF family. It has been previously demonstrated that FGF21 is a potent regulator of glucose homeostasis. To improve stability of FGF21 for better efficacy, a new form of recombinant FGF21 was generated by fusion of a full length FGF21 gene and the Fc fragment of human IgG4 with flexible linker sequence. To examine the glucose regulation activity of FGF21-L-Fc, 3T3-L1 pre-adipocytes were differentiated into adipocytes, and glucose uptake activity of FGF21-L-Fc was examined by glucose oxidase and peroxidase (GOD-POD) assay. The results showed that in comparison with wild type FGF21, FGF21-L-Fc was more potent in stimulation of glucose uptake by 3T3-L1. In vivo studies on the modified protein demonstrated that FGF-L-Fc had a better efficacy in lowering blood glucose of the STZ-induced diabetic animals and controlled glucose level for a longer time. The results provided a sound basis for further studies.


Asunto(s)
Animales , Masculino , Ratones , Células 3T3-L1 , Adipocitos , Metabolismo , Glucemia , Metabolismo , Diabetes Mellitus Experimental , Quimioterapia , Metabolismo , Escherichia coli , Metabolismo , Factores de Crecimiento de Fibroblastos , Genética , Farmacología , Glucosa , Metabolismo , Inmunoglobulina G , Genética , Farmacología , Proteínas Recombinantes , Genética , Farmacología
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