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China Journal of Chinese Materia Medica ; (24): 523-525, 2005.
Artículo en Chino | WPRIM | ID: wpr-279122

RESUMEN

<p><b>OBJECTIVE</b>To establish a method for determination of galanthamine in Lycoris radiata.</p><p><b>METHOD</b>HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.</p><p><b>RESULT</b>Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).</p><p><b>CONCLUSION</b>The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.</p>


Asunto(s)
Cromatografía Líquida de Alta Presión , Métodos , Galantamina , Lycoris , Química , Raíces de Plantas , Química , Plantas Medicinales , Química , Control de Calidad
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