Determination of Concentration of Dextromethorphan Hydrobromide in Human Serum by HPLC / 中国药房

OBJECTIVE:To establish a HPLC method for determination of concentration of dextromethorphan hydrobro?mide in human serum.METHODS:The assay was conducted on HiQsil C 18 column(4.6mm?150mm,5?m)with acetonitrile-water-acetic acid(35∶63∶2,V/V/V)as mobile phase.The fluorescence detector measured with the excitation wavelength of280nm and the emission wavelength of320nm,and carvedilol was taken as internal standard.RESULTS:The linear range of calibration curve of dextromethorphan hydrobromide was0.27～21.90ng/ml with a regressive equation of Y=0.1197X+0.0086,r=0.9986.The recovery was high.CONCLUSION:This method is accurate,rapid and simple,and can be used for de?termining the concentration of dextromethorphan hydrobromide in human serum.