Comparison of Mesenchymal Stem Cell Markers in Multiple Human Adult Stem Cells

OBJECTIVES: Mesenchymal stem cells (MSCs) are adult stem cells which identified by adherence to plastic, expression of cell surface markers including CD44, CD90, CD105, CD106, CD166, and Stro-1, lack of the expression of hematopoietic markers, no immunogenic effect and replacement of damaged tissues. These properties led to development of progressive methods to isolation and characterization of MSCs from various sources for therapeutic applications in regenerative medicine. METHODS: We isolated MSC-like cells from testis biopsies, ovary, hair follicle and umbilical cord Wharton's jelly and investigated the expression of specific cell surface antigens using flow cytometry in order to verify stemness properties of these cells. RESULTS: All four cell types adhered to plastic culture flask a few days after primary culture. All our cells positively expressed common MSC-specific cell surface markers. Moreover, our results revealed the expression of CD19and CD45 antigens in these cells. CONCLUSION: According to our results, high expression of CD44 in spermatogonial stem cells (SSCs), hair follicle stem cells (HFSCs),granulosa cells (GCs)and Wharton's jelly-MSCs (WJ-MSCs)may help them to maintain stemness properties. Furthermore, we suggest that CD105+SSCs, HFSCs and WJ-MSCs revealed the osteogenic potential of these cells. Moreover, high expression of CD90 in SSCs and HFSCs may associate to higher growth and differentiation potential of these cells. Further, the presence of CD19 on SSCs and GCs may help them to efficiency in response to transmembrane signals. Thus, these four types of MSCs may be useful in clinical applications and cell therapy.

[Growth inhibitory and differentiating effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, in human myeloid leukemia K562 cell line]

Because drug resistance is one of the most important problems in patients with chronic myeloid leukemia [CML], finding new anti-cancer drugs especially from natural sources is research priority. Therefore, in this study, anti-cancer effects of ethyl acetate soluble metabolite of Iranian native bacteria, Streptomyces calvus, were studied using human chronic myeloid leukemia K562 cells. In this experimental trail, ethyl acetate soluble metabolites were isolated from S. calvus bacteria. K562 cell line was treated by various concentrations of this metabolite for 12- 72 h intervals. Anti-proliferative effects of ethyl acetate soluble metabolite were studied by trypan blue exclusion test. Wright-Giemsa staining and latex particle phagocytosis assay were used to study differentiated cells. Ethyl acetate soluble metabolite induced growth inhibition in K562 cells in concentration and time- dependent manner. At the concentration of 200 ng/ml, the growth was inhibited 19-50% after 12-72h. Latex particle phagocytosis assay and Wright- Giemsa staining results revealed that K562 cells were differentiated toward monocytic- macrophagic lineage. According to growth inhibitory and differentiating effects of S.calvus metabolite in K562 cells, this metabolite can be proposed for more investigations in differentiation therapy of CML patients.