RESUMEN
Pomegranate juice has several antioxidant components such as flavenoids and mineral materials such as sodium and potassium. In this study the effects of pomegranate juice on bone calcium content and body weight of adult mice were survived. In this applied study two doses [3.3 and 6.6 ml/kg] of pomegranate juice [PJ] were gavaged to female mice for 30 days. Animals were weighed at days of 0, 5, 10, 15, 20, 25 and 30. Bone calcium contents were measured by flame photometer. Bone calcium content of PJE treated mice increased but it was not significant statistically. Pomegranate juice did not affect body weight. Pomegranate juice extracts even its high dose did not show any side effect on body weight and tissues of adult female mice
RESUMEN
To evaluate the effects of Prunus domestica L. extracts on fetuses and neonatal skeletal systems. A total of 32 pregnant mice [Mus musculus] received vehicle and plum hydroalcoholic extract at gestational days 1-18 and during the entire gestational period as well as 10 days postpartum, respectively. A total of 30 nonpregnant mice were fed plum hydroalcoholic extract and plum juice extract for 30 days. Bone calcium content and serum concentrations of calcium, magnesium and alkaline phosphatase were measured. The skeletal systems of their fetuses and neonates were stained with Alcian blue and alizarin red S and the length of femur, tibia, and their ossification center were measured. Crown-rump length of the newborn mice from mothers treated with plum extract [4.61 +/- 0.25 mm] was higher compared to the control group [4.48 +/- 0.31 mm, p = 0.001], and the femur osteogenesis index of newborn mice from mothers treated with plum extract was also higher [0.87 +/- 0.09] compared to the control group [0.81 +/- 0.06, p = 0.007]. The findings showed that pregnant mice treated with plum extract had fetuses and newborn mice with higher osteogenesis index than those of the controls