RESUMEN
Background: Family of colony-stimulating factors [CSF] have an essential role on early cross talk between embryo and uterine endometrium
Objective: The aim of this study was to evaluate the effects of the single dose of Granulocyte-CSF [G-CSF] injection on clinical outcome of assisted reproductive technology cycle in patients with repeated implantation failures
Materials and Methods: This randomized control trial study was performed on 52 infertile women who referred to the clinic with the history of more than three previous In vitro fertilization/Intracytoplasmic sperm injection-embryo transfer failures. All patients were stimulated with standard long protocol. All embryos were transferred on day five in blastocyst stage in both groups. The treated group received 300 Mug [0.5 ml] recombinant human G-CSF subcutaneously which was injected 30 min before blastocyst embryo transfer
Results: There was not statistically significant differences in abortion rate in G-CSF and control group [p=0.09]. G-CSF treated group showed higher clinical pregnancy rate in comparison with control group [56.2% vs. 40.0%] but it was not statistically significant [p=0.09]. Although live birth rate in G-CSF group was higher than control group [53.1% vs. 35.0%] but there wasn't statistically significant difference in the overall live birth rate between the two groups [p=0.10]. G-CSF group had a twin pregnancies while in control group there was no twin pregnancy
Conclusion: Our result demonstrates the possibility that pregnancy outcome is better in women with repeated unexplained In vitro fertilization failure who are treated with G-CSF
RESUMEN
Background: Natural endometrium in Frozen-thawed Embryo Transfer [FET] may have some benefits upon implantation in patients with Repeated Implantation Failure [RIF]. It might be due to possible differences between natural and stimulated endometrial growth factors and cytokins secretions
Objective: The objective of this study was to compare the pregnancy rate of FET on modified natural cycle versus hormone replacement therapy [HRT] cycle endometrium in patients with RIF
Materials and Methods: In this observational study the pregnancy rate of patients with RIF undergoing day 3 FET in natural cycle endometrium [group 1, n=56], were compared with another group of patients with RIF in whom frozen-thawed day 3 embryos were transferred on HRT cycle [group 2, n=52]
Results: The pregnancy rate in group 1 was 41.07%, compared with the pregnancy rate of group 2; 36.5% [p=0.63]. The abortion rate was not significantly different among the groups
Conclusion: It can be concluded that FET in a modified natural cycle is comparable with HRT cycle in patients with RIF
RESUMEN
Nuclear transfer-embryonic stem cells [NT-ESCs] are genetically identical to the donor's cells; provide a renewable source of tissue for replacement, and therefore, decrease the risk of immune rejection. Trichostatin A [TSA] as a histone deacetylase inhibitor [HDACi] plays an important role in the reorganization of the genome and epigenetic changes. In this study, we examined whether TSA treatment after somatic cell nuclear transfer [SCNT] can improve the developmental rate of embryos and establishment rate of NT-ESCs line, as well as whether TSA treatment can improve histone modification in NT-ESCs lines. In this experimental study, mature oocytes were recovered from BDF1 [C57BL/6xDBA/2] mice and enucleated by micromanipulator. Cumulus cells were injected into enucleated oocytes as donor. Reconstructed embryos were activated in the presence or absence of TSA and cultured for 5 days. Blastocysts were transferred on inactive mouse embryonic fibroblasts [MEF], so ESCs lines were established. ESCs markers were evaluated by reverse transcription-polymerase chain reaction [RT-PCR]. Histone modifications were analyzed by enzyme linked immunosorbent assay [ELISA]. Result of this study showed that TSA treatment after SCNT can improve developmental rate of embryos [21.12 +/- 3.56 vs.8.08 +/- 7.92], as well as establishment rate of NT-ESCs line [25 vs.12.5]. We established 6 NT-ESCs in two experimental groups, and three embryonic stem cells [ESCs] lines as control group. TSA treatment has no effect in H3K4 acetylation and H3K9 tri-methylation in ESCs. TSA plays a key role in the developmental rate of embryos, establishment rate of ESC lines after SCNT, and regulation of histone modification in NT-ESCs, in a manner similar to that of ESCs established from normal blastocysts