Spectrophotometric and spetrofluorimetric methods for determination of rutin in presence of ascorbic acid

Two simple, sensitive and specific methods for the determination of rutin in tablets in presence of ascorbic acid are presented. The first method depends upon measuring the first derivative response of rutin in methanolic/NaOH solution at 430 nm, where ascorbic acid showed no interference. The second method utilized the fluorescence characteristics of rutin/AlCl3 complex in methanol, where ascorbic acid has no contribution. The validity of the proposed methods was proved by analysis of synthetic mixtures of rutin and ascorbic acid and have been applied successfully to the analysis of rutin in tablets