Fermentation with microorganism for indigo / 中成药

AIM: To apply the natural fermentation technology to produce indigo. METHODS: Fresh leaves of conehead were put in water and let its ferment naturally, then add lime to fermentation liquor to naturalize,indigo content was determined by HPLC. RESULTS: Fermentative temperature at 28 ℃ and oxygen supply were most important condition. CONCLUSION: Fungi can be successfully applied to indigo production.